凯氏定氮法测N元素.doc

凯氏定氮法测N元素 采用凯氏消煮法用硫酸钾-硫酸铜为加速剂，其时间虽然较长，但只要控制好加速剂用量，不易导致氮素损失，消化程度容易掌握，测定结果稳定，准确度较高。消煮后氮的测定用蒸馏法。测定结果由北京林业大学生物中心提供。 1材料与方法 材料 取新鲜草莓叶片，编号称重，95°C烘干后研磨至粉末状。 1.2 仪器 凯式定氮仪KDY-9820 试剂 400g/L氢氧化钠溶液，甲基红-溴甲酚绿混合指示剂，20g/L硼酸溶液，20g/L硼酸-指示剂溶液，0.02mol/L1/2Na2B4O7标准溶液，1mol/L盐酸溶液，0.02mol/L盐酸标准溶液。 方法 消煮：用浓硫酸消化样品 全氮含量 A1 3.2884% A2 3.3018% A3 3.2513% A4 3.0729% A5 0.2221% b1 3.0167 b2 2.7785 b3 2.5330 b4 2.4976 说明1=2# Albion 2=2# San Andreas 3=3# Albion 4=3# San Andreas 5=3# soil A 12月 B1月 2#=2号温室 3#=3号温室 Kjeldahl determination to measure?N?elements Kjeldahl Digesting Method using potassium sulfate and copper sulphate for accelerating agent consumes longer time, but it is difficult to lead to nitrogen loss, easy to know the digesting extent, stable with measurement results and high accuracy as long as the amount of control the accelerator. The nitrogen after digesting is detected by distillation method. Determination results were made in the biological center of Beijing Forestry University. 1 ?Materials and methods 1.1 Materials Fresh strawberry leaves were weighed,?we pestle them into powder after 95℃?drying?(1?to?2 #?Albion,?2?to?2 #?San Andreas,?3?to?3 #?Albion,?4?to?3 #?San Andreas) Instruments Kjeldahl apparatus?KDY-9820 1.3 Reagents 400g / L?sodium hydroxide solution, methyl red?-?bromocresol green mixed indicator,?20g / L?boric acid,?20g / L?boric acid?-?indicator?solution, 0.02mol/L1/2Na2B4O7?standard solution,?1mol / L?hydrochloric acid solution,?0.02mol / L?hydrochloric acid standard solution. 1.4 Methods Digestion: samples digested with concentrated sulfuric acid,?potassium sulfate?-?copper sulfate as accelerator. Distillation: to?250mL?conical flask?15mL20g / L?boric acid?-?indicator solution, the flask placed in fixed nitrogen distillation unit to undertake the condenser tube, the tube port placed in boric acid solution at the surface of 3-4cm.?The solution containing all of the Kjeldahl digestion flask in the distillation unit installed, open the co