山莨菪碱对大鼠心肌缺血再灌注损伤心功能的影响_精品.pdfVIP

山莨菪碱对大鼠心肌缺血再灌注损伤心功能的影响 陈彧泮、唐晓健、金晶、赵怡君、陈治文 指导老师：周冠怀 陈红 王莉萍 [摘要] 目的 旨在探讨山莨菪碱对大鼠离体心肌缺血再灌注损伤的影响。 方法 20 大鼠离体心脏采用 Langendorff 灌流法随机分为四组，对照组、山莨 菪碱小剂量组 (20mg/L)和大剂量组 (100mg/L )、NO合酶抑制剂L-NAME加大剂 量山莨菪碱组。稳定 10 分钟后，停灌 30 分钟，再复灌 30 分钟，观察心功能指 标及冠脉流量的变化。结果 山莨菪碱可使缺血再灌注心脏复跳时间和室颤时程 缩短， LVP 增高、LVEDP 降低、dP/dt 和-dP/dt 均大于对照组（P0.05）冠脉流 量增加（P0.05），这些作用呈剂量依赖性且不能被 L-NAME 所拮抗。结论 山莨 菪碱可以改善缺血再灌注损伤大鼠的心功能，这种保护作用不是通过影响 NO 合 成所致。 [关键词] 山莨菪碱；心肌缺血；再灌注损伤 The effect of anisodamine on isolated heart ischemia/reperfusion injury in rats Chen yupan,Tang xiaojian, Jin jing, Zhao yijun, Chen zhiwen Shanghai Second Medical University (SSMU), Shanghai 200025, CHINA [Abstract] Objective: To study The effect of anisodamine on isolated heart ischemia/reperfusion injury in rats.Method: Tweanty Wistar rats were allocated into four groups:control Low Anisodamine(20mg/L) ,High Anisodamine(100mg/L),L-NAME(6mg/L)+High Anisodamine. Hearts were perfused in a Langendorff setup for 10 minutes before subjected to 30 minutes ischemia and 30 minutes in reperfusion.Cardiac function and coronary flow were recorded.Results:Anisodamine reduced recovery time and ventricular fibrillation duration,left ventricular pressure augment,left ventricular end-diastolic pressure diminish,dP/dt and –dP/dt are more than the control group,coronary flow augment(P0.05).These effects were dose-dependant and can’t be antagonized by L-NAME.Conlusion:Anisodamine ameliorate the funtion of the heart 1 ischemia/reperfusion injury in rats.Cardioprotection is NO-indenpendant. Key words:Anisodamine,heart ischemia,reperfusion injury 80年代以来，随着危重病急救医学和心脏病学的发展，人们已经了解到， 冠脉再通术、脏器移植、休克、脑复苏和多脏器功能衰竭等救治过程中都存在再