Critical Review of Published Microarray Studies for Cancer Clinical 癌症临床发表的微阵列研究述评.ppt

下载文档 降价啦

3
0
约8.98千字
约 44页
2018-04-17 发布于江西
举报
版权申诉
保障服务

Critical Review of Published Microarray Studies for Cancer Clinical 癌症临床发表的微阵列研究述评.ppt

1、本文档共44页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

Guidelines on Statistical Analysis and Reporting of DNA Microarray Studies of Clinical Outcome Richard Simon, D.Sc. Chief, Biometric Research Branch National Cancer Institute Bethesda Maryland USA BRB Website/brb Powerpoint presentations and audio files Reprints Technical Reports BRB-ArrayTools software BRB-ArrayTools Data Archive Sample Size Planning for Targeted Clinical Trials Publications Reviewed Searched Medline Hand screening of abstracts papers Original study on human cancer patients Published in English before December 31, 2004 Analyzed gene expression of more than 1000 probes Related gene expression to clinical outcome Types of Clinical Outcome Survival or disease-free survival Response to therapy 90 publications identified that met criteria Abstracted information for all 90 Performed detailed review of statistical analysis for the 42 papers published in 2004 Good Microarray Studies Have Clear Objectives Class Comparison (Gene Finding) Find genes whose expression differs among predetermined classes, e.g. tissue or experimental condition Class Prediction Prediction of predetermined class (e.g. treatment outcome) using information from gene expression profile Survival risk-group prediction Class Discovery Discover clusters of specimens having similar expression profiles Class Comparison and Class Prediction Not clustering problems Supervised methods Major Flaws Found in 40 Studies Published in 2004 Inadequate control of multiple comparisons in gene finding 9/23 studies had unclear or inadequate methods to deal with false positives 10,000 genes x .05 significance level = 500 false positives Misleading report of prediction accuracy 12/28 reports based on incomplete cross-validation Misleading use of cluster analysis 13/28 studies invalidly claimed that expression clusters based on differentially expressed genes could help distinguish clinical outcomes 50% of studies contained one or more major flaws Do’s Don’tsGeneral Do define and state the objecti