Efficient Environment, Part II Phenol Resins：高效的环境，第二酚树脂.pptVIP

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Efficient Environment, Part II Phenol Resins：高效的环境，第二酚树脂.ppt

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Efficient Environment, Part II Phenol Resins：高效的环境，第二酚树脂.ppt

Efficient Environment, Part II:Phenol Resins Structure of Phenol Resins Many phenolic resins are composed of formaldehyde and phenols Sub-project 1: Phenol metabolism Goal: Engineer E. coli to metabolize phenol as a carbon source, linking it to cellular respiration Meta- and Ortho- Phenol Metabolism Sub-Project 2: Formaldehyde Metabolism Goal: Engineer E. coli to metabolize formaldehyde as respiration precursor, bypassing the detoxification pathway Oxidation versus assimilation Project in Context Tsai et al. isolated a strain of yeast capable of degrading both phenol (meta-fission) and formaldehyde, but acknowledged that “a microbe with a biodegrading power toward phenol and formaldehyde concurrently has been scarcely reported” (Tsai et al. 2005) Gusse et al. isolated white-rot fungus capable of degrading phenolic resins but efficiency uncertain (Gusse et al. 2006) Hidalgo et al. showed the ability of Rhodococcus erythropolis UPV-1 to grow on phenol and remove formaldehyde Linking both to cellular respiration (not just detoxification) in E. coli would facilitate future projects to make PR degradation more efficient--more genetically tractable and better understood Questions to Consider Can we make phenol the sole or preferred carbon source? Competition with formaldehyde resistance pathway? Linking to other pathways/phenomena in bacteria? (e.g. quorum sensing, biofilms, etc.) * Tuah 2006. A schematic representation of the central carbon metabolism of E. coli (adapted from ref. 35) Shlomi T. et.al. PNAS 2005;102:7695-7700 ?2005 by National Academy of Sciences Phenol as a Carbon Source By-products of phenol metabolism would enter glycolysis pathway or TCA Cycle of E. coli The sequences of the enzymes for both ortho- and meta- pathways are available online /fulltext/ms2001058/fig1.gif 3-Hexulose-6-Phosphate Synthase: Condensation of ribulose 5-phosphate with formaldehyde to form 3-hexulose 6-phosphate. phospho-3-hexuloisomerase: 3-hexulose 6-phosphate to form D