DrySlidetechnology干化学技术.ppt

Dry Chemistry干化学 Variable Dry Chemistry 干化学之种类 Tablet 药片剂 Baxter Paramax, Dupont Dimension Strip 试纸条 Ames Multi-Film 多层薄膜 Ortho Vitros System, FujiChem True Dry Chemistry 真正之干化学Multi-Coating Slide 多涂层薄膜干试剂片 Dry Slide 干片Methodology方法学 Components of One Slide干片之基本组成 Spreading Layer分布扩散层 Uniformly distributes the sample 均匀分布标本 Spreading Layer 分布层 Uniformly distributes the sample 均匀分布标本 Filters out large molecules 过滤大分子 May contain reagents to break up molecules: by physical or by chemical 可包含打断大分子的试剂：物理性和 化学性 A background for reflective purpose 提供反射测定的背景 A Background for Light Reflection光反射的背景 Reagent Layer 试剂层 Contains reagent in dry form 包含干性试剂 Control each reaction sequence 控制反应顺序 Indicator Layer 指示剂层 Drive reaction toward completion 将反应驱于完成 Contains a dye to produce a colored complex 包含染料以产生显色复合物 Support Layer 支持层 Provide for a supporting base 提供反应支持基 Allows lights to pass through 允许光路通过 Types of Slides干片类型 Potentiometric Slides电势电位法干片 ISE, Ion Selective Electrode Technology 离子选择法电极技术 3 tests are performed on Vitros System Sodium ( Na+) 钠离子 Potassium (K+) 钾离子 Chloride (Cl-) 氯离子 Measures the potential difference between the two electrodes 测定两电极间电势的不同 Chemical PotentialandSelectivity Immuno-Rate Slides免疫－速率法干片 Examples of IR tests: 免疫速率法干片举例： Digoxin, Phenytoin, Phenobarbital, CRP 地高辛，苯妥英，苯巴比妥，C－反应蛋白 Competitive Non-competitive IR format 竞争与非竞争性免疫速率反应模式 Liquid substrate is required 需要液体反应底物 Application of Sample应用举例 Gelatin Reagent Competitive Binding 竞争性结合 Binding Ag Free Ag 结合抗原 与 游离抗原 Gelatin Reagent Substrate Addition 底物加入 Gelatin Reagent Determine Analyte Conc.分析物测定 Low Conc. High Conc. Determine Analyte Conc. 分析物测定 (Competitive Method 竞争性方法) Dry Slide Technology 干化学技术 Spreading layer 分布扩散层 Reagent layer 试剂层 Indicator layer 指示剂层 Support layer 支持层 Sample 标本 Spreading layer 分布层 Reagent layer 试剂层 Indicator layer 指示层 Support layer 支持层 Uniformly distribute the sample 均匀分布标本 Un