石墨炉原子吸收培训课程之二.pptVIP

Rapid Furnace Heating快速加熱爐台 當瞬間達到原子化溫度時可提高感度 快速加熱達到原子化溫度可提高原子雲團密度 The final temperature must be controlled, however, since there are losses at high temperatures due to the rapid expansion of the gas within the tube In PerkinElmer instruments, max.power heating occurs at rates of 2000oC/sec. and the temperature is controlled by an optical sensor that has been calibrated to the desired temperature. Maximum Power HeatingTime-Temperature Profiles Maximum Power HeatingEffect on Sensitivity Gas Flows in a HGA Atomizer氣體流速 Internal and External Gases內,外氣流 Argon Nitrogen: may be used instead of Ar but reacts with carbon above 2300oC to produce toxic cyanogen gas Oxygen or air: useful in ashing samples in situ that may be high in organic matter; ashing temperature usually restricted to 600oC or lower Argon/Hydrogen: 95%Ar/5% H2 often useful in eliminating chloride interference; see EPA Method 200.9 Furnace Setup Procedure石墨爐AAS設定步驟 1. Install and align lamps 2. Install a new graphite tube and platform (if applicable) 3. Align the furnace in spectrometer (generally, for instruments that can be converted from flame to furnace operation) 4. Condition the graphite tube 5. Align the autosampler 6. Run blanks Furnace Setup Procedure (續) 7.Run sensitivity checks 8.Calculate characteristic mass (mo) THGA Furnace 爐台 Furnace Alignment (HGA Systems)石墨爐調整位置 At PerkinElmer, Cu is often used as the element to check furnace alignment and sensitivity Instrument mode is in continuous readout, with an integration time of approx. 0.5 sec., and the background corrector is off The furnace is removed from the optical path and the instrument is zeroed Place the furnace in the optical path and adjust the furnace alignment as necessary The absorbance should be 0.1 Abs. New Tube Installation新石墨管安裝 Wipe the interior of the contact cylinders清理爐台內之管壁 Lint-free cloth such as Kim Wipe Cotton Swab often moistened with DI water or methanol Condition the tube最佳化石墨管 Condition file pre-programmed in