Metastasis Detection in Sentinel Lymph Nodes Comparison of a Limited Widely Spaced (NSABP protocol B-32) and a Comprehensive Narrowly Spaced Paraffin Block Sectioning Strategy文档.pdf

NIH Public Access Author Manuscript Am J Surg Pathol . Author manuscript; available in PMC 2010 December 22. N Published in final edited form as: I H Am J Surg Pathol . 2009 November ; 33(11): 1583–1589. doi:10.1097/PAS.0b013e3181b274e7. - P A A u Metastasis Detection in Sentinel Lymph Nodes: Comparison of a t h o r Limited Widely Spaced (NSABP protocol B-32) and a M a n Comprehensive Narrowly Spaced Paraffin Block Sectioning u s c r Strategy i p t Donald L. Weaver, MD1,4,5, U. Phuong Le, MD1,5, Stacey L. Dupuis, BS, Katherine A. E. Weaver, Seth P. Harlow, MD2,4,5, Takamaru Ashikaga, PhD3,4, and David N. Krag, MD2,4,5 1Department of Pathology, University of Vermont, Burlington, Vermont 2Department of Surgery, University of Vermont, Burlington, Vermont 3 N Department of Biostatistics, University of Vermont, Burlington, Vermont I H- 4Vermont Cancer Center, Burlington, Vermont P A 5Fletcher Allen Health Care, Burlington, Vermont A u t h Abstract o r The NSABP B-32 trial is examining whether patients with initially negative sentinel lymph nodes M (SLNs) who have occult metastases detected on deeper levels and cytokeratin a n immunohistochemistry (CK-IHC) stains are at risk for regional or distant metastases. The u s experimental B-32 protocol was designed to detect metastases larger than 1.0 mm by examining c r sections approximately 0.5 and 1.0 mm deeper into the paraffin blocks (2 levels; wide spacing). i p t This pilot quality assurance study compares detection rates to a comprehensive protocol designed to de