干旱胁迫对楝叶吴茱萸春花任豆中MDASOD-林业与环境科学.DOC

干旱胁迫对华南地区三种苗木膜脂过氧化及保护酶活性的影响 潘昕 李吉跃(摘要 本文研究了华南石灰岩地区常见植物任豆，以及两种石灰岩地区造林供选树种楝叶吴茱萸、石斑木在干旱胁迫下体内丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化物酶（POD）活性的变化，为在石灰岩地区筛选优良的抗旱节水树种提供了科学的理论依据。试验结果表明,三个树种在干旱胁迫下都表现出MDA含量先升高后降低的趋势，其中楝叶吴茱萸在干旱胁迫第9 d最先达到峰值（5.08 ?mol），其次是任豆在第12 d达到最高值4.46 ?mol，石斑木峰值含量最，在第12d达到3.35 ?mol。苗木SOD活性也都表现出先增大后减小的规律，在中度干旱时期达到峰值，石斑木在第12 d达到517.40 Ug。POD活性呈先升高后降低再升高的趋势，石斑木另两种植物比较，第一个峰值更迟出现，第二个峰值POD活性最高，在第24 d达到1 490.24 mg·g·min-1。经过对三项指标的综合分析并结合植物的抗旱性表现，三个树种中石斑木的适应性较强，抗旱性能较好，其次是任豆，楝叶吴茱萸的抗旱能力较差。 关键词 干旱胁迫MDA SOD POD 楝叶吴茱萸石斑木任豆 Drought Stress on Membrane Lipid Peroxidation and Protective Enzyme Activity of Three Tree Species Seedlings in Southern China Pan Xin Li Jiyue* Su Yan He Qian Qiu Quan Lin Wen (College of Forestry, South China Agriculture University, Guangzhou, 510642) Abstract The research is about the changes of malondialdehyde (MDA) content, superoxide dismutase (SOD) activity and peroxidase (POD) activity in a common plant that Zenia insignis which in limestone regions of Southern China,and two kinds of limestone area afforestation for selected tree species in Evodia meliaefolia (Hance) Benth. and Rhaphiolepis indica Lindl, under drought stress. It provides significant theoretical support and reference for the selection and breeding of fine drought-resistant and water-saving tree species in the limestone area. The test results show, after drought treatment,three tree species under drought stress demonstrated a trend that their MDA content increased at first and then decreased. The MDA content of Evodia meliaefolia (Hance) Benth. turned out to be the highest, reached the maximum value of 5.08 ?mol/gFW in the 9th day first, Zenia insignis followed it,reached 4.46 ?mol/gFW in the 12th day,and Rhaphiolepis indica Lindl contained the lowest,reaching 3.35 ?mol/gFW in the 12th day.Seedlings’s SOD activity also shows a trend of increasing first and then decreasing, reaching its peak in the moderate drought period. Rhaphiolepis indica Lindl was in the hig