造影剂微泡的制备及其在高频超声下兔子老鼠体内的应用Preparation.PDF

Vol. 4 No.1/ Jan.Vol. 4 No.1/ Jan. 010010 造影剂微泡的制备及其在高频超声下 兔子老鼠体内的应用 Preparation of microbubbles and their application in rat and rabbit under high frequency ultrasound 靳巧锋　钱明　牛丽丽　郑海荣 摘 要 目的：为高频超声成像系统开发用于检查小动物的最佳超声造影剂微泡。材料和方法：将全氟丙烷CF气体包裹 3 8 在磷脂分子构成的外壳中形成造影剂微泡，用PSS780A颗粒计数器测量微泡的粒径分布，微泡的粒径主要分布在0.8μm， 其数量模式和中值粒径分别为0.67和0.83μm。用Vevo770高频超声系统外接的 RMV-707B 30兆赫的超声探头来评价造影 剂微泡的造影效果。首先用模拟血管在体外进行了实验；在大鼠体内注射造影剂微泡的前后分别观察了其颈总动脉及颈动 脉分叉位置的造影，另外还观察了兔子体内注射造影剂微泡后其颈动脉及后腿的动静脉部位造影。结论：粒径不到1μm 小 微泡适合做30兆赫的高频超声成像系统的造影剂。 关键词 超声；微泡；高频；vevo770 ABSTRACT Purpose: To develop optimal microbubble contrast agents for high frequency ultrasound imaging systems when examining small animals. Materials and Methods: We prepared microbubbles by encapsulate (Perfluoropropane) CF into phospholipids shell, and the size distribution of the microbubbles was measured by PSS 0A particle sizing systems. A major peak was observed in the size distribution of the microbubbles at 0.8 μm with mode and median of 0.67 and 0.83 μm, respectively. Vevo 770 system coupled with RMV-707B 30MHz transducer was used to evaluate the quality of the microbubbles. Experiment in vitro by using blood vessel mimic was carried out. Carotid and carotid bifurcation contrast images before and after injecting microbubbles was clearly observed in live rat. Carotid and back leg artery and vein was observed in live rabbit. Conclusion: small size microbubbles less than 1μm is suitable as contrast agent for 30 MHz high frequency ultrasound imaging systems. KEYWORDS ultrasound; microbubbles; high frequency ;vevo 0 1 I