2 5己二酮致人卵巢颗粒细胞凋亡及sonichedgehog信号通路调控机制的研究 word格式.docx

目录学位论文原创性声明和版权使用授权书··································1 缩略词表···························································2 中文摘要···························································3ABSTRACT7正文·······························································12 前言····························································12 第一部分人卵巢颗粒细胞分离、原代培养与鉴定体系的建立引言························································18 材料与方法··················································18 结果························································24 讨论························································28第二部分2,5-己二酮致人卵巢颗粒细胞凋亡机制的研究引言························································31 材料与方法··················································32 结果························································45 讨论························································56第三部分Sonichedgehog信号通路在2,5-己二酮致人卵巢颗粒细胞凋亡过程中的调控作用引言························································61 材料与方法··················································62 结果························································65 讨论························································76 小结····························································79 参考文献························································81 附件····························································89 致谢·······························································92 综述·······························································93 攻读学位期间主要研究成果··········································108缩略词表(按字母顺序排列)缩写词BAX英文全称BCL-2associatedXprotein中文名称BCL-2相关X蛋白BCL-2Bcelllymphoma/lewkmia-2B细胞淋巴瘤/白血病-2CASPASEcysteinylaspartate-specificprotease半胱氨酸-天冬氨酸特异蛋白酶cDNAcomplementaryDeoxyribonucleicacid互补脱氧核糖核酸DEPCdiethypyrocarbonate焦碳酸二乙酯DMEMDulbecco’smodifiedEaglemediumDMEM培养液FITCfluoresceineisothiocyanate异硫氰酸酯GCgranulosacell颗粒细胞HCGhumanchorionicgonadotropin人绒毛膜促性腺激素2,5-HD2,5-hexanedione2,5-己二酮ICSIintracytoplasmicsperminjection卵胞浆内单精子注射IVF-ETinvitrofertilization-embryotr