arac在酵母菌 白血病细胞和aml小鼠模型中的抗性分析 word格式.docxVIP

StudyofAra-C ResistanceinYeasts,AMLCells andMouseModelAbstractConclusions:PBtransposonsystemcaneffectivelyinsertedintheyeastgenome, whichmakesitservesasaneffectivemutagenesistoolforgeneticscreens.The mutationsanddecreasedexpressionsofDCKinAMLresistantcellsmaybean importantreasonforAra-Cchemotherapyresistance.Thecombinedtreatmentwith Ara-CandABT-737suppressedleukemiaandprolongedthesurvivaltoanevengreater extentthanthegrouptreatedwithAra-Calone,whichindicatsthatresistantleukemic cells retain oreven upregulatetheirintrinsic anti-apoptotic mechanisms.Keywords: PBtransposon; Schizosaccharomycespombe;AML;DCK;ABT-737Writtenby:Wenjun ZhaoSupervised by:Prof. BinYinIII目录引言································································································1材料与方法··························································································4一、材料·······················································································41. 细胞株················································································42. 实验药品·············································································43. 主要试剂·············································································44. 实验仪器·············································································55.主要实验耗材········································································6二、实验方法·················································································61. 主要试剂的配制····································································62 主要实验方法········································································8实验结果···························································································161.YHL912 酵母菌株生长曲线及对G418的敏感性检测··························16讨论······························································································23参考文献···························································································25综述Ara-C 代谢相关基因对AML