人蜕膜细胞体外培养体系的建立 copy.pdf

实用预防医 2007 年 10 月 第 14 卷 第5 期 Practical PreventiveMedicine, Oct. 2007, Vol 14,No. 5 1335 文章编号: 1006- 3110(2007)05- 1335- 03 = 论 著 人蜕膜细胞体外培养体系的建立 1 2 1 2 2 2 , , , , , : 改良人蜕膜细胞( decidual cell) 的培养方法, 并对其进行特征鉴定, 探讨蜕膜细胞的部分生物 特性。 取人工流产6~ 8 周妊娠蜕膜组织, 机械法分离, 复合酶消化, 传代自然增殖法纯化细胞。倒置显微镜观察其大 体形态及体外转化,组织 鉴定、HE 染色观察其胞核情况,人催乳素(HPL)免疫组化法鉴定。流式细胞仪测定其细胞周 期。 分离培养的蜕膜细胞呈长梭形和不规则星形,95%细胞胞浆阳性表达。细胞周期显示约有59.8% 的细胞处 于G / G 期。 成功分离培养了人蜕膜细胞, 方法经济、简单、获得细胞形态一致、成活率高、生长稳定, 为进一步 0 1 研究蜕膜细胞妊娠病理、生理改变提供了可靠的实验对象。 : 滋养层细胞; 免疫细胞化学; 细胞培养; 细胞周期 中图分类号: 32912 文献标识码:A 1 2 1 Establishment of VitroCulture System of Human Decidual Cell LI U Xiao- li , QIN Ming- chun , YU Ai- ming , et al. ( 1. Haici Hospital of Qingdao City, Qingdao 2660332, handong; 21 Hunan University of TCM, Changsha 410007, Hu2 nan) Abstract: Objective To improve the method for isolation and culture ofhuman decidual cell, and to investigate its par2 tial biological properties. Methods Human decidual tissues were taken from pregnant women of 6~ 8 weeks with induced abortions, then digested bycomplex enzyme and purified through severalnatural replication passages. The cellular morpholo2 gy was observed by inverted phase contrast microscope and the nucleus and cytoplasm were observed by staining ofhaema2 toxylin and eosin (HE). ecidual cellwas identified by the method ofimmunocytochemistryofprolactin (PRL). Cellcycle was observed by flow cytometry. Results The cultured cells appeared as fusiform or irregular astroform. Protein ofPRL was expressed in 95% cultured cell