[医学]研究生分子生物学工作基础 蛋白质相互作用研究方法.pptVIP

Two-hybrid screens are low-tech; they can be carried out in any lab without sophisticated equipment. Two-hybrid screens can provide an important first hint for the identification of interaction partners. The assay is scalable, which makes it possible to screen for interactions among many proteins. Yeast two-hybrid data can be of similar quality to data generated by the alternative approach of coaffinity purification followed by mass spectrometry (AP/MS). Y2H Strengths Y2H Weaknesses A high number of false positive (and false negative) identifications. The hybrid prote