贺福初院士人类肝脏蛋白质组计划的实施和毒理学发展的机遇.ppt

Outline Background Progress of the HLPP Initiation of the CNHLPP Next work Research projects Distribute the Chinese specimens to reference labs outside China Establish the SOPs for the black racial people`s liver sample Comprehensively analyze the data of the proteins expressing profile of the French specimens Establish the SOPs for the protein-protein interactions and ORFeome Initiate virtually diseased liver proteome subproject Initiate virtually proteomic modification and localization subprojects Coordination Continue to discuss the cooperation with other initiatives (e.g. MRPP, HPPP, PSI, HAI, etc.) or other programme (e.g. Systems Biology Project of Germany) Establish the headquarters of the HLPP Continue to propagandize the HLPP to the public and private domains Strive for more financial supports from more resources It is a giant ambitious objective and gives a great challenge to embryonic proteomics It is necessary to carry out a preview before the formal launching of this project Science 302:1316, Nov. 21, 2003 Nature 425: 441, Oct. 2, 2003 Average Throughput of the Platform 2DE System 12 Gel / 3 Days Auto-Spot Digest System 1152 spots / Day MS and MS/MS System 500-1000 Spots / Day The flowchart of CCPIT for the protein expression profile of human fetal liver The area scales before and after isoform processing in group and protein levels respectively. In protein level, the red circularity areas represent the number of confirmed proteins and the yellow annulus areas represent the number of possible proteins. Non-iso groups 19% Extensively confirmed 81% Groups 41% Confirmed 59% Non-isoforms 43% Isoforms 57% c o n t r i b u t e 5 4 5 a d d i t i o n a l p r o t e i n s a n d e l i m i n a t e 7 6 3 g r o u p s c o n t r i b u t e 5 4 5 a d d i t i o n a l p r o t e i n s a n d e l i m i n a t e 1 3 3 5 p o s s i b l e p r o t e i n