The Effects of pH and Temperature on the Germination of ：温度和pH对种子萌发的影响.ppt

The Effects of pH and Temperature on the Germination of Raphanus sativus Seeds Keara Miley – Lake Shore Robin Smith – Lake Shore Terminology Two-Factor Design of Experiment (DOE) Raphanus sativus (Radish) Germination When a seed starts to sprout pH How alkaline or acidic a substance is How does pH affect germination? pH affects germination because the acid breaks down the seed coat and allows more water to get absorbed Temperature affecting Germination If temperature is too high, moisture evaporates from the soil If temperature is too low, water absorption slows Why Do The Experiment? More areas we can grow food, the more food we can grow The purpose of the experiment was to find the pH level and temperature that would cause the most Raphanus sativus (radish) seeds to germinate Problem How do different levels of pH and temperature affect the germination of radish seeds? Hypothesis If the surrounding temperature is 35oC and the pH level of the water sprayed on the seeds is 4.0, then the most radish seeds will germinate Values Materials Source of tap water Analytical balance 100 mL spray bottles Paper towels Ziploc bags Incubator (35°C) Refrigerator pH test strips tweezers Procedure We mixed the water with either baking soda or citric acid to change the pH and get a high, a low, and a standard concentration We sprayed the concentrations onto the radish seeds placed in Ziploc bags Procedure We then placed the bags into areas with the different temperature levels After 3 days, we recorded the number of seeds that germinated in a table Procedure Data Analysis The effect of pH on the germination of radish seeds was -0.17 units On average, as pH increased, the number of seeds germinated decreased by 0.17 units Data Analysis The effect of temperature on the germination of radish seeds was 0.5 units On average, as temperature increased, the number of seeds germinated increased by 0.5 units Data Analysis There may be an interaction between the two line segments becau