儿童分离金黄色葡萄球菌对12种常用抗生素耐药性分析[精品].docVIP

儿童分离金黄色葡萄球菌对12种常用抗生素耐药性分析 ? 论看?JMedRes,Dee2007,Vo1.36No.12 儿童分离金黄色葡萄球菌对12种 常用抗生素耐药性分析 李珊华春珍徐立群 摘要目的了解临床分离的金黄色葡萄球菌儿童株对常用抗生素的耐药特点以及与成人株的耐药性差异.方法对 2004年2月～2005年6月分离的185株儿童临床株和2002年9月一2003年5月分离的74株健康携带株进行了耐药模式研究, 并与成人患者中分离的39株金葡菌进行耐药性对比,同时用E—test法进行l2种常用抗生素的药敏试验.结果259株金黄色 葡萄球菌儿童株中,98.5%产生B内酰胺酶而对青霉素耐药,91.1%的菌株对苯唑西林敏感,91.9%对头孢噻肟和头孢曲松敏 感,对红霉素,四环素,甲氧苄啶～磺胺甲基异恶唑,氧氟沙星的耐药率分别为48.3%,30.9%,11.2%,10.0%和2.3%;成人株对 苯唑西林,头孢噻肟,头孢曲松,红霉素,四环素,甲氧苄啶一磺胺甲基异恶唑,氧氟沙星的耐药率分别为69.2%,66.7%,66.7%, 89.8%,69.3%,6.1%和64.1%,差异有显着性意义(Plt;0.05);所有菌株均对万古霉素敏感.结论本研究中金葡菌对苯唑西 林耐药率不高,对绝大部分抗生素的敏感率显着高于成人株. 关键词金黄色葡萄球菌儿童成人耐药性 StudyonDrug—resistanceofStaphylococcusAureusIsolatedfromChildreninHangzhou.LiShan,HuaChunzhen,XuLiqun.Depart— mentofPediatrics,HangzhouNo.2PeopleSHospital,HospitalAffiliatedtoHangzhouNormalUniversity;Zh4iang310015,China Abstract0bjectiveTostudytheantimicrobialagentsresistanceofStaphylOCOCCU~$aureus(S.aureus)isolatedfromchildrenand compareitwiththatofstrainsisolatedfromadultsinHangzhou.MethodsS.aureusisolateswerescreenedaccordingtolatexagglutina?tiontestandidentifiedwithGPIcardofViteksystem.Antibioticssensitivitytestwereperformedusingdiskdiffusionmethodsandsensitiv?itytestofoxacillinandvancomycinwereperformedwithafurtherE梩estmethod.ResultsOfall259S.aureusstrains,247strains (95.8%)wereB?lactamase梡ositiveandresistanttopenicillin,while91.1%ofallstrainsweresensitivetooxacillin,91.9%ofall strainsweresusceptibletoeefotaximeandceftriaxone.Resistanceratestoerythromycin,tetracycline,trimethoprim梥ulfamethoxazole, andofloxacinwere48.3%,30.9%,11.2%,10.0%and2.3%respectively.Theresistanceratetooxacillin,cefotaxime,ceftriaxone, erythromycin,tetracycline,trimethoprim梥ulfamethoxazole,andofloxacininadultsstrainswere69.2%,66.7%,66.7%,89.8%, 69.3%,6.1%and64.1%respectively,whichweresignificantlyhigherthanthatinchildrenstrains(Plt;0.05,al1).ConclusionsOx— acillin—resistanceinS.aureusisolateswaslow,andtheresistanceratesofS.aureustocommonantibioticswerehigherthanthatina— duhsstrains. KeywordsStaphylococcusaureus;Ch