含有缬氨酸和精氨酸的抗菌肽及其活性-中国科技论文在线.PDFVIP

中国科技论文在线 含有缬氨酸和精氨酸的抗菌肽及其活性 # 马清泉，王国红* （东北农业大学动物科技学院，哈尔滨，150030 ） 5 摘要：目的: 抗菌肽分子主要由包含疏水性残基和阳离子性残基在内的残基组成。本研究的 抗菌肽采用Schiffer-Edmundson 螺旋轮模型推导而来，使得抗菌肽具有两亲性的特点。其中， 由带正电荷的精氨酸构成螺旋模型的亲水一侧，由疏水氨基酸缬氨酸构成疏水一侧，设计了 一系列抗菌肽。方法:采用美国临床实验室标准化研究所推荐的方法测定最小抑菌浓度；还 10 使用红细胞测定最小溶血浓度。结果:在电荷数保持不变的情况下，随着疏水性的增强，抗 菌肽的抑菌活性呈现出增加的趋势;在模拟细菌细胞膜环境，肽呈现α-螺旋结构。结论:本研 究成功设计出了主要由缬氨酸和精氨酸构成的全新抗菌肽，并筛选出了细胞选择性高的分 子。 关键词：动物营养与饲料科学；抗菌肽；螺旋；生物活性 15 中图分类号：S828 Valine- and Arginine-rich antimicrobial peptides and their activities MA Qingquan, WANG Guohong 20 (Animal Science Department, Northeast Agricultrual University, Harbin 150030) Abstract: Antimicrobial peptides are mainly composed of hydrophobic residues and positively charged residues. These peptides were derived from Schiffer-Edmundson wheel projections. The peptides were designed by segregating positively charged amino acid (Arg) in the hydrophilic side and hydrophobic amino acid (Valine) in the hydrophobic side. Minimum inhibitory concentration and minimum 25 hemolytic concentration were tested. Results showed that the increase of the hydrophobicity of the peptides linearly increased antibacterial activity without influence on hemolysis and cytotoxicity. Collectively, we successfully designed a series of de novo peptides with valine and arginine in this study. Key words: animal nutrition and feed science; antimicrobial peptides; helix; biological activity 30 0 引言 抗菌肽分布广泛，在各种生物体内都先后发现了不同数量的抗菌肽，包括细菌、真菌、 昆虫、鸟类、鱼类、哺乳动物、植物在内的所有生物体都可产生抗菌肽[1]。抗菌肽具有抑 35 制和杀死细菌、抗真菌