树龄和性别对天然东北红豆杉中紫杉醇及三尖杉宁碱质量分数的影响.doc

树龄和性别对天然东北红豆杉中紫杉醇及三尖杉宁碱质量分数的影响 第39卷第12期 2011年12月 东北林业大学 J0URNALOFN0RTHEASTF0RESTRYUNIVERSITY V01.39No.12 Dec.201l 树龄和性别对天然东北红豆杉中紫杉醇及 三尖杉宁二大丁碱质量分数的影响 刘彤常醉王洋 (东北林业大学,哈尔滨,150040)(东北油田盐碱植被恢复与重建教育部重点实验室/盐碱地生物资源环境研究中心(东北林业大学)) 周志强 (森林植物生态学教育部重点实验室(东北林业大学)) 摘要在黑龙江穆棱东北红豆杉自然保护区采集了不同树龄,不同性别的天然东北红豆杉(Taxuscmpidata Sieb.etZucc.)当年生和2年生枝,利用反相高效液相色谱对108株东北红豆杉(胸径d在1.8～72.2cm之间)枝 中紫杉醇及三尖杉宁碱质量分数进行测定分析.结果表明:随树龄增加,紫杉醇质量分数呈先上升后下降的趋势, 而三尖杉宁碱质量分数随树龄增加而降低.≤15am和dgt;60cm径级组内,雄株紫杉醇质量分数高于雌株;15cmlt; d~lt;30am径级组内,雌株的紫杉醇质量分数明显高于雄株.≤15em径级组内,雌株三尖杉宁碱质量分数高于雄 株,此后雄株三尖杉宁碱质量分数相对于雌株增加更多;在dgt;60am径级组内,雄株紫杉醇质量分数高于雌株.总 体上,在dgt;30am之后,雌雄株的紫杉醇和三尖杉宁碱质量分数变化趋势一致. 关键词东北红豆杉;树龄;性别;紫杉醇;三尖杉宁碱 分类号$718.43- DifferencesinC0ntentsofPaclitaxelandCephalomannineinTaxuscuspidateofDifferentAgesandSexes/LiuTong (SchoolofForestry,NortheastForestryUniversity,Harbin150040,P.R.China);ChangZui,WangYang(KeyLabora— toryofSaline—AlkaliVegetationEcologyRestorationinOilField(NortheastForestryUniversity),MinistryofEducati0n/ AlkaliSoilNaturalEnvironmentalScienceCenter):ZhouZhiqiang(KeyLaboratoryofForestPlantEcology(Northeast ForestryUniversity),MinistryofEducation)//JoumalofNortheastForestryUniversity.一2011,39(12).一51～53.76 Current—yearneedlesandbiennialneedleswerecollectedformTaxuacusDf(10ofdifierentagesandsexesinMuling NatureReserveofHeilongjiangProvince.Thecontentsofpaclitaxelandcephalomannineintheneedlesweremeasuredwith HPLC.Resaltsshowedthattheaveragecontentofpaclitaxelintheneedlesfirstincreasedandtl1endecreasedwithincreasing age(diameteratbreastheight(DBH)ran~ngfrom1.8amto72.2cm);whiletheaveragecontentofcephalomanninede. creasedwithincreasingage.Theaveragecontentofpaclitaxelinthemaleplantsofc~pidatawashigherthanthatinthe femaleoneswithDBHlessthan15amormorethan60cm:whilethefemaleplantscontainedhigherpaelitaxelthanthe maleoneswithDBHrangingfrom15amto30am.Theaveragecontentofcephalomannineinthefemaleplantswashigher thanthatinthemaleoneswithDBHlessthan15em.andtheaveragecontentofpaclitaxelinthemaleplantsgraduallyin- creasedwithincreasi