玄黄药膏对小鼠急性软组织损伤组织中IL-6影响实验研究汇总.pdfVIP

摘 要 目的：通过实验研究玄黄药膏对小鼠急性软组织损伤模型 IL-6 含量的影响，为该药 的作用机制、疗效、临床规范应用及推广提供依据。 方法：1、 建立小鼠急性筋伤（急性软组织损伤）模型：KM种小鼠90只，体重（20 ±2 ）g，雌雄各半，实验前适应性3d，采用角叉菜胶致炎法造模，给予玄黄药膏进行 治疗，同时设凡士林空白组及正骨水对照组； 2 、取造模成功后1d、3d、5d、7d 各组中分别处死3 只小鼠，用手术刀切开小鼠炎 症部位皮肤，分离皮下组织，切取损伤部位中心处呈青紫色的组织，用生理盐水漂洗 1min，滤纸吸干，分析天平称取 (100±5)mg，每10 mg 组织加入生理盐水0.5ml，于 匀浆器中缓慢而均匀地研磨15min，至匀浆液呈均匀一致悬浊液，倒入试管中在高速 冷冻离心机以2500r ·min-1 离心15min，收集上清液，置于-20 ℃冰箱中备用。用ELISA 法测定IL-6 的含量。实验结果根据标准品的浓度及对应的OD 值，计算出标准曲线的 回归方程，再根据样本的OD 值，在回归方程上计算出对应的样品浓度，也可以使用 应用软件来计算。最终浓度为实际测定浓度乘以稀释倍数。 结果：玄黄药膏处理组能明显抑制小鼠组织内IL-6 含量。用药 1 天后，玄黄药膏组 和正骨水组的 IL-6 含量无明显差别，玄黄药膏组与凡士林组的 IL-6 含量无明显差别, 使用方差分析，结果 P0.05,样本间无显著性差异。用药3、5、7 天后，玄黄药膏组 和正骨水组的 IL-6 含量无明显差别，P0.05 有统计学意义；方差分析认为这两样本 间有显著性差异。 玄黄药膏组与凡士林组的 IL-6 含量差别明显，P0.05 有统计学 意义；方差分析认为这两样样本间有显著性差异。 结论：玄黄药膏具有明显抑制损伤组织的IL-6 含量。 关键词：玄黄药膏；急性软组织损伤模型；IL-6 。 Xuan Huang ointment on acute soft tissue injury Experimental study on the effect of IL-6 mice Abstract Objective: To by experimental research Xuan Huang ointment on acute soft tissue injury model IL-6 content, provide the basis for the drugs mechanism of action, efficacy, clinical practice application and promotion. Method: 1, the establishment of acute tendon injury (acute soft tissue injury) model: 90 KM mice, body weight (20 ± 2) g, male and female, before the experiment adaptability 3d, carrageenan-induced inflammation lawmodeling, given the mysterious Xuan Huang ointment for treatment, located Vaseline blank group and bone water control group; 2, take modeling and after treatment 1d, 3d, 5d, 7d group were sacrificed mice, cut with a scalpel sites of inflammation in mice skin, subcutaneous tissue was separated, cut at the center of the injury site was purple organizations withthe saline rinse 1min, dry filter paper, weighed analytical balance (100 ± 5) mg per 10 mg of tissue was added 0.5 ml of saline in the homogenizer slowly and evenly grinding for 15 min