一个“基质加工肽酶”调控叶绿体和根的发育 [PCP].pdfVIP

Plant and Cell Physiology Advance Access published January 22, 2010 Running Title: Rice stromal processing peptidase *Corresponding author Tel: +86 571 Fax: +86 571 E-mail: clspwu@ Subject areas Growth and development Number of black and white figures, color figures and tables Figures: 7 figures including 5 color figures and 2 black and white figures Supplementary materials: 2 tables © The Author 2010. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@ a rice stromal processing peptidase regulates chloroplast and root development Runqing Yue, Xiaofei Wang, Jieyu Chen, Xiaoxia Ma, Huanhuan Zhang, Chuanzao Mao, Ping Wu* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China Abbreviations: AR, adventitious root; GFP, green fluorescent protein; LR, lateral root; MT, mutant; SPP, Oryza Sativa stromal processing peptidase; TEM, transmission electron microscopy; WT, wild type Abstract The stromal processing peptidase (SPP) is a metalloendopeptidase that cleaves a broad range of precursor substrates. In this study, we isolated a rice mutant showed leaf chlorosis at early seedling stage but inhibition of root growth during the whole growth period. Genetic analysis demonstrates that the phenotypes of the mutant were caused by a recessive single gene mutation. The mutated gene was cloned by map-based cloning, which encodes a stromal processing peptidase (SPP). Sequence analysis showed a Glu deletion in highly conserved C-terminus of SPP in the mutant. The mutation of SPP in the mutant was verified by transgenic complementation. SPP is constitutively expressed in all tissues. The subcellular localization analysis indicates that SPP is targeted into chloroplast. The expression of so