土壤放线菌ⅲ-172次级代谢产物的分离、纯化及结构鉴定.pdfVIP

Abstract Shanghai Normal University Master of Philosophy Abstract A batch of mutant strains producing ascomycin provided by Shanghai Pesticide Research South Center, were fermented and fostered separately, then a series of procedures were dealt with them. During the process, a lot of biological activity tests were done to them, their antimicrobial activities and the growth condition of strain on the slant were confirmed as two screening indicators, finally the soil actinomycete Ⅲ-172 was obtained, which can produce metabolites with strong inhibitory activity against plant pathogens. In this paper, the antimicrobial ingredients of soil actinomycete Ⅲ-172 were isolated and purified. Before fermentation, some culture conditions were simply optimized such as the fermentation time and organic solvent extraction. By comparing the inhibition activity, the fermentation time was determined as 7 days, and n-butyl alcohol was chosen as the best organic solvent. Through fermentation with the optimized conditions, high- speed centrifuge, solvent extract, silica gel chromatography, thin layer chromatography, and high performance liquid chromatography and other ways were used, combined with the tracking test of the biologic activity of plant pathogen, ascomycin chosen as a comparison standard to isolate and extract the active substances from its metabolites. When the ratio of expansion agent was CH Cl :CH OH=10:1, and the Rf value was about 0.43,one active strip signed strip 5 2 2 3 was detected on the TLC plate. High performance liquid chromatography (HPLC) analysis was carried out on the strip 5. Finally one seperate active peak c