黑水虻幼虫肠道和体表产酶细菌的分离和鉴定.pdf

昆虫知识 Chinese Bulletin of Entomology 20 10 ，47 (5 ):889 ～ 894 黑水虻幼虫肠道和体表产酶细菌的 分离和鉴定* 1 1 ，2 1 ，2 1 ，3 4 1  喻国辉 牛春艳 何国宝 周 林 夏 嫱 程 萍 (1. 珠海市农业科学研究中心 珠海 5 19075 ;2. 中山大学生命科学学院 广州 5 10275 ; 3. 华中农业大学生命科学学院 武汉 430070 ;4 . 遵义医学院珠海校区 珠海 5 1940 1) Isolation and identification of bacteria producing enzymes from gut and skin of black soldier fly (H ermetia illulens )larvae. YU Guo-Hui1 ，NIU Chun-Yan1 ，2 ，HE Guo-Bao1 ，2 ，ZHOU Lin1 ，3 ，XIA Qiang4 ， CHENG Ping1  (1. Zhuhai Agriculture Research Center ，Zhuhai 5 19075 ，China ;2. Dep artment of Lif e Science ，Sun Yat-sun University ，Guangzhou 5 10275 ，China ;3. Dep artment of Lif e Science and Technology ， Huazhong Agricultural University ，Wuhan 430070 ，China ;4 . Zunyi Medical College Zhuhai Camp us ，Zhuhai 5 1904 1 ，China) Abstract Ten bacterial strains were isolated from the gut ，or skin ，of the black soldier fly (Hermetia illucens L . )larvae ，collected from the field or when feeding indoors. The strains from the gut were named S14 ，S15 ， S16 ，S19 and S20 ，and those from skin were named T2 ，T4 ，T6 ，T7 and T8 . All strains had both protein and organic phosphate metabolic capability . The 16 S rDNA sequences of these strains were cloned ;BLAST analytical results identified all strains as Bacillus species. The gyrB gene ，which encodes DNA gyrase B protein ，was used to identify the strains to the species level ;all strains were identified as B. subtilis . A phylogenetic tree based on the gyrB gene sequences of the ten strains ，B. subtilis subsp . subtilis str . 168 and strain BCRC10255 ，indicates that the ten B. strains have phylogenetic