损伤血瘀证微观细胞凋亡、免疫机制和中医宏观思维诊断的比较分析-中医诊断学专业论文.docx

Purpose:AbstractThe topic to be from apoptosis, immune cells the perspective of injuryresearch stasis for the future from a clinical point of skeletal muscle apoptosis, immune injury blood stasis point of effective diagnosis and a reasonable grasp of different pathological cycle of drug use and provide experimental period room basis. Through exploration and research of this thesis, not only for the injury blood stasis syndrome make an important contribution to the objective, and more able to TCM Syndrome supplement the scientific content of the unity of the macro and micro, local and global unity ，static and dynamic unity.Method:80 SD rats were randomly assigned into 8 groups, namely control group, 2h after the modeling group, 8h after the modeling group, 24h after the modeling group, 2d， 3d ，5d ，7d group after the model, n = 10. Using sophisticated modeling of quantitative methods to combat gravity, the control group before modeling, and the remaining groups were as in the corresponding time points rats were killed, and isolated from skeletal muscle cells and blood tests apoptosis of skeletal muscle cells, pathological biopsy and blood indicators of immune cytokines IL-2, IL-4, IL-6, IL-8, IL-10 and TNF-α, dynamic observation of apoptosis in skeletal muscle after injury, pathological states, changes in systemic immune through injury early stasis various objective of the experimental data to determine evidence of injury the nature of early blood stasis. Theory combined with traditional Chinese medicine diagnosis, through the systematic comparison of Chinese and Western medicine, Chinese medicinediagnostics to explore the macro and micro, local and global, static anddynamic unity of the theoretical basis.Results:AP-TUNNEL TESTED calculate early injury (1 week or less) the level of the basic laws of the apoptosis.Caspase-3 measured by flow cytometry staining and cell apoptosis rate with a high degree of correlation.Cytokines IL-2 clear trends to form a curve; IL-4 ris