土生空团菌的培养特性及其对宿主植物促生作用研究-微生物学专业论文.docx

Study on Culture Characteristics and the Growth-promoting on Host Plants of Cenococcum geophilumAbstractThe best propagation conditions and the Growth-promoting on Ostryopsis davidiana and pinus tabulaeformis of the five strains of Cenococcum geophilum (CgSO1, CgSB2, CgO5, CgSPOP2 and Cg5#) was studied in this paper. The single-factor tests, orthogonal tests and liquid confirmatory test were used to research the cultuer characteristics of the 5 Cg strains. The growth-promoting on O. davidiana and P. tabulaeformis of the 5 Cg strains were studied.The main results were following:The optimized culturing conditions of 5 Cg strains were determined separately. The results showed that the 5 Cg strains had different nutrition physiological characteristics. The optimum pH, carbon source, nitrogen source, microelements components, mineral salt components, vitamin requirement were different excepted the optimum temperature; The biomass of the 5 Cg strains were increased significantly. The monoclony biomass of 5 Cg strains were 0.0643g, 0.0853g, 0.0985g, 0.0659g and 0.0891g; The results of the corresponding liquid cultivation were 13.189 g/L, 12.413 g/L, 18.852 g/, 11.511 g/L and 14.179 g/L.The results showed that the infection rates of the 4 Cg stains (CgSO1, CgSB2, CgO5 and CgSPOP2) on the O.davidiana were 43.5%, 46.8%, 49.2% and 48.1% respectively. However, there were only CgSO1 and CgSPOP2 which could form the mycorrhiza structure with P.tabulaeformis, and the infection rates were just 10.3% and 12.4%. After inoculating Cg, the heights of mycorrhizal seedling of O.davidiana were 49.6% to 65.9% higher than the control. The ground diameters were increased by 14.9% to 21.9%. The first grade lateral roots were increased by 21.7% to 34.8%. The shoots dry weights were raised by 51% to 69.9%. The roots dry weights were raised by 108% to 135.5%. The total dry weight was raised by 71.8% to 88.3%. The root/shoot ratios were increased by 27.1% to 46%. The relative water contents were