五组趋化轴在骨髓间充质干细胞向大鼠缺血区心肌趋化作用比较-心血管病学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 五组趋化轴在骨髓间充质干细胞向大鼠缺血区心肌趋化作用比较 中文摘要 个/HPF（P0.05），加入 AMD3100 组的心梗 1 天缺血区心肌条件培养基组对 BMSCs 迁移数减少为 32.20±6.140 个/HPF，心梗 3 天缺血区心肌条件培养基组对 BMSCs 迁 移数减少为 26.6±4.278 个/HPF，心梗 7 天缺血区心肌条件培养基组对 BMSCs 迁移数 减少为 34.2±7.369 个/HPF。 结论： 1、缺血缺氧心肌条件培养基中培养 BMSCs，其 CXCR4、CCR2、CX3CR1 表达 量随培养时间增加较对照组持续高表达，而 c-met 及 CCR1 表达量较对照组明显降低。 2、心梗后大鼠非缺血区心肌 SDF-1、MCP-1、HGF、Fractalkine 及 CCL7 表达较 正常组心肌无显著性差异，而缺血区心肌 SDF-1、MCP-1、HGF、Fractalkine 及 CCL7 表达较正常组心肌呈现时序性变化。 3、BMSCs 向心梗后缺血区心肌趋化过程中 SDF-1/CXCR4 较其他 4 组趋化轴趋 化能力可能更强。 关键词：骨髓间充质干细胞，大鼠，心肌梗死，趋化轴 作 者：王志松 指导老师：惠 杰 II II 英文摘要 五组趋化轴在骨髓间充质干细胞向大鼠缺血区心肌趋化作用比较 The comparison of the five chemotactic axis which cause BMSCs to migrate to myocardial ischemic area of rat Abstract Objective：To compare the effect of five chemotactic axis on BMSCs migrating to ischemic myocardium of SD rat. Methods： 1. According to the differently adherent characteristic of cells, we isolated and cultured BMSCs from the tibia and femur of aged 8 weeks SD rats. And the passage 3th cells were used to the experiment. we simulated the cardiac microenviroment in vitro by adding myocardial cell lysate into the culture of BMSCs. BMSCs cultured in normoxic serum-containing myocardial conditioned medium or in serum-free and mionectic myocardial conditioned medium for 6h, 12h, 18h, 24h and 48h. CXCR4, CCR1, CCR2, CX3CR and c-met expression of BMSCs were measured by real time PCR. 2. We ligated the branch of coronary artery by thoracotomy to establish myocardial infarction model weighing 250g. We divided the rat heat into two parts, namely ischemic myocardium and non-ischemic myocardium, through injection of 3% of the Evens blue in the inferior vena cava. Expression of SDF-1, MCP-1, CLL7, Fractalkine and HGF were measured by RT-PCR or real time PCR of this two parts for 1, 2, 3, 7, and 14 days after infarction. 3.Transwell chemotactic experiment: according to the results of the experiment which indicated CXCR4, CCR2, CX3CR1 expressing higher continuously when BMSCs cultured in