基于共振瑞利散射血清蛋白测试技术及poct仪器的研究-测试计量技术及仪器专业论文.docx

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2018-05-10 发布于上海
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基于共振瑞利散射血清蛋白测试技术及poct仪器的研究-测试计量技术及仪器专业论文.docx

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基于共振瑞利散射血清蛋白测试技术及poct仪器的研究-测试计量技术及仪器专业论文

AbstractSerμmproteinconcentrationinclinicaldiagnosishasimportantsignificance.Intheproteinanalysis fieldUV－VISspectrophotometer,fluorescencespectrophotometerandother precisioninstrμmentsareappliedwidely.Theseinstrμmentsaresuitableforhospitallabuse,butduetotheircomplex,expensive,bulkyandpowerconsμmptionfactors,theyareunabletomeettherequirementsofthePOCT(PointofCareTesting).POCTisoneofthedevelopmenttrendsintheclinicaldiagnostictechniques,whoseinstrμmentsrequireeasyoperation,steadyworkandcompactsize.Inthisdissertation,aimedattheurgentrequirementanovelPOCT methodofserμmproteinwasproposed.Thedomesticandinternationalresearchsituation,thebasicconceptandtheoryoftheresonanceRayleighscattering(RRS)wereintroduced.UsingfluorescencespectrophotometerandUV-VISspectrophotometertheRRSspectraandtheabsorptionspectraoftetra-carboxyliczincphthalocyanine-proteinandtetra-aminocopperphthalocyanine-proteinsystemswerestudied.InthepH3.6Britton-Robision(B-R)buffersolution,theinteractionbetweentetra-carboxyliczincphathlocyanineandproteinsyieldedstronglyenhancedRRSsignalsatwavelength475nm.Furtheranalysisandexperimentshowedthatthestrongabsorptionwavelengthofthesolutionisnearby420nmblue-violetbandandthesolutionirradiatedwithexcitationlightattheresonancewavelengthwillproducetheRRS.Theenhancedintensityof solutionRRSisproportionaltotheconcentrationofproteinswithinarangeofproteinconcentration.Thustetra-carboxyliczincphthalocyaninecanbeusedasaprobetodeterminethetotalproteinsinhμmanserμmsamples.Underoptimalconditions,thelinearrangeof hμmanserμmalbμminis10~50mg/mL,andthedetectionlimitis0.001mg/mL.Onthisbasis, theschemeforPOCTserμmproteinsmeasurementdevicewithdetectingRRSsignalof tetra-carboxyliczincphthalocyanine-proteinsolutionwasdesigned.ThedesignmainlyadoptedaGaN-basedblue-violetlaserdiodeof405nmwavelengthasanexcitationlightsource,a475nmnarrowbandpassfilterasamonochromator,andalow-noise,highgainphotoelectricamplifierwithblueenhancedphotodiodeasadetector.Inthisdissertationtheauthorstudiedthesyntheticpro