脂肪干细胞和间充质干细胞体外诱导分化为心肌细胞比较.pdfVIP

生理学报 Acta Physiologica Sinica, June 25, 2008, 60 (3): 341-347 341 研究论文 脂肪干细胞与间充质干细胞体外诱导分化为心肌细胞的差别 1 2,* 2 张端珍 ，盖鲁粤 ，刘宏伟 1 2 沈阳军区总医院先心病内科，沈阳 110016 ； 解放军总医院心内科，北京 100853 摘 要：本文旨在探讨脂肪干细胞(adipose-derived stem cells, ASCs)和骨髓间充质干细胞(mesenchymal stem cells, MSCs)在组 织含量、体外培养和诱导分化为心肌细胞方面的差别。ASCs 从新西兰白兔皮下脂肪组织提取，MSCs 从大鼠四肢长骨骨 髓提取，体外培养扩增，免疫细胞学方法鉴定。采用细胞集落形成法检测组织中干细胞的含量。将不同代的干细胞用不 同浓度的5- 氮胞苷诱导，观察其形态变化，免疫细胞化学方法检测诱导后细胞是否转化为心肌细胞。结果显示，体外培 养的ASC s 呈短梭形，分布均匀，生长迅速，细胞形态单一、稳定。M SC s 原代生长非常缓慢，呈簇生长，细胞纯度 偏低，容易混杂其它细胞类型，传代细胞容易分化和老化。脂肪组织中ASCs 含量显著高于骨髓中MSCs 含量，且前者 含量受年龄影响小。5- 氮胞苷诱导ASCs 分化为心肌细胞的有效浓度为6~9 μmol/L ，而MSCs 在3~15 μmol/L 5- 氮胞苷诱 导下可见心肌细胞形成。ASCs 诱导分化的心肌细胞呈球形细胞团，MSCs 分化的心肌细胞呈条形或棒状，其心肌细胞分 化率低于ASCs 。幼年动物MSCs 的组织含量和心肌细胞分化率均高于老年动物，而ASCs 受动物年龄影响较小。结果表 明，ASC s 在组织含量、细胞纯度、生长速度和心肌细胞分化率等方面均明显优于骨髓MSC s ，在心肌细胞再生方面较 MSCs 具有更大的优势。 关键词：成体干细胞；细胞分化；心肌细胞 中图分类号：Q254 ；R329.28 Differences between adipose-derived stem cells and mesenchymal stem cells in differentiation into cardiomyocytes 1 2,* 2 ZHANG Duan-Zhen , GAI Lu-Yue , LIU Hong-Wei 1Department of Congenital Heart Disease, the General Hospital of Shenyang Military Region, Shenyang 110016, China; 2Depart- ment of Cardiology, the Chinese PLA General Hospital, Beijing 100853, China Abstract: Adipose-derived stem cells (ASCs) are similar to bone marrow mesenchymal stem cells (MSCs) in growth kinetics, antigen expression and multi-lineage differentiation capacity. The present study was designed to investigate the differences between ASCs and MSCs in in vitro culture and differentiation into cardiomyocytes. ASCs were isolated from the fat tissue of New Zealand white rabbits while MSCs were obtained from rat bone marrow. Both ASCs and MSCs were cultured in Iscove’s modified Dulbecco’s medium supplemented with 15% fetal bovine seru