不同应激强度对小鼠衰老进程影响差异的研究.pdfVIP

98 7 1 11 不同应激强度对小鼠衰老进程影响差异的研究 马　虹　田苏平　陈启盛 　研究不同强度应激对小鼠衰老改变影响的差异。　对于 - 半乳糖进行衰老诱导的小鼠同步进行不 D 同 温( 12℃、18℃、25℃) 游泳应激训练, 连续六周后, 进行开场行为、学习记忆能力、脑MDA 含量及海马SOD 活性测定。 　18℃及 25℃ 温应激训练有对抗D- 半乳糖所致行为和学习记忆能力减退的作用( P 0.01) , 同时脑中抗氧化能力提 高( P 0.05) ; 而12℃ 温组学习记忆能力进 一步减退, 海马SOD 活性进 一步下降( P 0.05) 。　应激对机体的影响具 有双重性; 适量的应激刺激可逆转机体的衰老改变, 提高动物的学习记忆能力, 而过强的应激刺激有加速衰老进程的倾向。两 种应激对机体抗氧化能力的不同改变可能是引起这 一差异的原因。 　应激强度　　D- 半乳糖　抗衰老　行为　SOD Diff erent ef fect in the aging process to mice by stress of dif f erent degree M a H ong , T ian S up ing , Chen Qisheng .J iang su staf f M ed ical Un iv ers ity 210029 A【bstract 】　Obj ective T o study different effect in t e Aging process to mice by stress of differdnt degree. Methods We took swimming stress at different t emperature as stress of different degree. W ile aging model w as made by D-galactose ip 0. 12 / / , 15 ( 12℃、18℃ 25℃) . mg g d for six w eeks t e mice sw am for min at different w ater temperature and T e open field be av- ior, t e ability of learning and memory and t e content of SOD and MDA in t e brains were measured. Results T e sw imming stress at 18℃and 25℃could increase ambulation and improve ability of discrimination and memory . but, sw imming stress at 12℃promoted aging process. Conclusions Stress serve a dual purpose: proper stress st imulus can incease t e activit y of an- . , tioxidase in brain and improve ability of learning and memory of aging mice In contrast excessive stress stimulus promotes ag- ing process. K【ey words 】　Stress st imulat ion　D-galactose　Antiaging Be avior　SOD : 50cm30cm, 21 , , , , , [ 1, 2, 4, 7] 3min 2. : Y , [ 5]