bmp2plga纳米羟基磷灰石缓释系统的制作及其体外实验研究word格式论文.docxVIP

中英文名对照与缩写缩写HA英文全名hydroxyapatite中文名称羟基磷灰石Nano-HAnanometer hydroxyapatite纳米羟基磷灰石ALPalkaline phosphatase碱性磷酸酶SEMscanning electronic microscope分析扫描电子显微镜BMP-2bone morphogenetic protein-2骨形成蛋白-2Adadenovirus vector腺病毒载体PCRpolymerase chain reaction多聚酶链反应RT-PCRreverse transcription polymerase逆转录多聚酶链反应chain reactionPAGEpolyacrylamide gel electrophoresis聚丙烯酰胺凝胶电泳SDSlauryl sodium sulfate十二烷基硫酸钠PBSphosphate buffer磷酸缓冲液DABdiarninobenzidine二氨基联苯胺ddH2O GFPdouble distilled water green fluorescence protein双蒸水 绿色荧光蛋白SEMscan electronic microscope扫描电镜BSAbovine serum albumin牛血清蛋白DMEMdulbeccos modified eagle medium细胞培养基DMSOdimethylsulfoxide二甲基亚砜FBSfetal bavine serum胎牛血清rpmrolls per minute每分钟转速Secsecond秒dday天ODoptical density光密度PLGApoly(1actic-co-glycolic acid聚丙交酯-乙交脂BMP-2/PLGA 纳米羟基磷灰石缓释系统的制作及其体外实验研究硕士研究生：李瑛导师：王大平 教授中文摘要目的：重组人工骨形态发生蛋白 2 (recombinant human bone morphogeneticprotein-2,rhBMP-2)在体内半衰期短，易发生稀释变性代谢，探讨利用聚丙交酯-乙交 酯共聚物（PLGA）复合纳米羟基磷灰石（nHA）制备载 rhBMP-2 微球的可行性及 制备工艺，并观察其载药、体外释药特性。方法：采用 rhBMP-2/纳米羟基磷灰石吸收法及 W/O/W 型复乳-溶剂交融技术制备 rhBMP-2/PLGA 纳米羟基磷灰石缓释微球，并对微球的粒径及形态、包封率、载药 量以及体外释放性质进行测定。结果：rhBMP-2/PLGA纳米羟基磷灰石缓释微球形态良好，粒径主要集中在50-70μm， 包封率为 (37.62±4.41)%，微球载药率为(5.22±1.42)%。微球内药物的释放量24小 时内超过15%，7d的释放量超过35%，于43天内释放大约90%。结论：制备的rhBMP-2/PLGA纳米羟基磷灰石缓释微球能缓慢持续的释放有活性的rhBMP-2，具有临床应用的可能性。【关键词】骨形态发生蛋白；聚丙交酯-乙交酯；组织工程；纳米羟基磷灰石；骨缺损；缓释 微球。广州医学院硕士学位论文英文摘要Preparation of nano hydroxyapatite BMP-2 loaded poly (1actic-co-glycolic acid) delivery microsphere and experimental study in vitroMaster Graduate: Ying Li Tutor: Prof. Daping WangABSTRACT【Objective】recombinant human bone morphogenetic protein-2(rhBMP-2) is prone to dilute、 denaturation and conduct metabolism, with a short half-life period in vivo.This study investigated the feasibility and the preparation of nano hydroxyapatite BMP-2 loadedpoly(1actic-co-glycolic acid) delivery microsphere, and observed drug-loading and drug-releasing function.【Methods】The rhBMP-2/PLGA/nHA microspheres were prepared using W/O/W solvent evaporation method. The particle size andmorph