brms1基因对卵巢癌细胞侵袭转移的影响及其机制分析word格式论文.docxVIP

The effect and mechanism of BRMS1 on the invasion and metastasis of human ovarian cancer cellsMajor: Gynecological OncologyMaster Degree Candidate:Zhou DongMei Mentor Professor：Prof. Sheng Xiu-jieDepartment of Obstetrics and Gynecology，the Third Affiliated Hospital of Guangzhou Medical University，Guangzhou, 510150, ChinaAbstractBackgroundOvarian cancer, a serious threat to the health of women, is the leading cause of death from gynecologic malignancies with a dismal poor overall 5-year survival rate (30％). With appeared insidiously, lacking of effective screening methods in early stage ,especaily proned to invasion and metastasis, so most patient were diagnosed in late stage, seriously affecting the prognosis of patients with ovarian cancer. Among them, invasion and metastatasis were to become the bottleneck for curing it. Currently, gene therapy has become the hotspot in the treatment of ovarian cancer research , and in animalexperiments and the Ⅰ, Ⅱ and Ⅲ clinical study it achieved a certain effect, which isexpected to become a new treatment model following the surgery, radiotherapy and chemotherapy. It was reported that , as one of the tumor metastasis suppressor gene, BRMS1 gene could inhibit a variety of tumors invasion and metastasis, including ovarian cancer. But because of its role is complex and atypical, and still less research on the specific mechanism , so in the cellular level studying the effect and possible mechanism of BRMS1 gene on the invasion and metastasis of ovarian cancer cells invitro , could explore a new target to control ovarian cancer invasion and metastasis for gene therapy, still more, clinical theoretical foundation will be established, so it will have certain scientific significance and potential clinical applications.ObjectiveUsing RNA interference technology, whit transient transfection, BRMS1 gene was silenced, by observing the changes of human ovarian cancer OVCAR3 cell invasion and metastasis , and de