deadbox rna解旋酶ddx25和ddx3x在登革病毒感染中的作用word格式论文.docxVIP

优秀毕业论文 精品参考文献资料 中文摘要 Dead-box RNA 解旋酶 DDX25 和 DDX3X 在登革病毒感染中的作用 DDX25 还能抑制 SeV,VSV 和 polyI:C 诱导的 IFN-β的转录。在 IFN-β信号通路中， DDX25 抑制由 RIG-I，MDA5，MAVS，TBK1，IRF3 过表达所诱导的 IFN-β的启 动子转录活性，与此同时，DDX25 也抑制了 RIG-I ，IKKα 和 p65 亚基过表达所 诱导的 NFκB 的转录活性。因此，我们得出结论，DDX25 通过 IRF3 和 NFκB 两条 支路抑制了 IFN-β的产生，促进了登革病毒的复制。 本研究首次证实了 DDX3X 的抗登革病毒功能；同时，也首次发现 DDX25 能 够抑制 IFN-β 信号通路，进一步丰富了 Dead-box 家族成员在抗病毒天然免疫中所 起到的作用，为抗病毒药物的研究提供了新的靶点。 关键词：登革病毒 DDX25 DDX3X IFN-β 作 者：李广豪 指导老师：戴建锋  PAGE 4 Dead-box RNA 解旋酶 DDX25 和 DDX3X 在登革病毒感染中的作用 英文摘要 Role of Dead-box RNA Helicase DDX25 and DDX3X During Dengue virus infection Abstract Dengue virus(DENV), transmitted by Aedes aegypti and Aedes albopictus, is the most widely distributed insect-borne virus in the world. It is a single strand RNA virus. Human beings, primate animals and mosquitos are the natural host of DENV. The severity of diseases can range from asymptomatic to life-threatening dengue hemorrhagic fevers (DHF) or dengue shock syndromes (DSS). Millions of DENV infections occur each year, but the mechanism of DENV infection is still not clear. DENV has 4 different serotypes. Infection with one type usually gives lifelong  HYPERLINK /wiki/Immunity_(medical) immunity to that type, but only short-term immunity to the others. Subsequent infection with a different type increases the risk of severe complications. For that reason, DENV vaccine development is challenging. Dead-box RNA Helicases belong to the large SF2 helicase superfamily. They participate in various processes of RNA metabolism. A number of DEAD-box helicases are involved in anti-viral immunity. In this project, an RNAi screening of 40 human DDX genes on DENV infected HEK293T cells were performed to investigate which DDXs are involved in DENV replication. DDX3X and DDX25 were selected as our research objects. The result showed that DENV replication was significantly upregulated in DDX3X siRNA-treated cells, but downregulated in DDX25 siRNA-treated cells. In order to further s