fe3o4sio2纳米粒子担载漆酶后的cu2修饰及酶学性质研究word格式论文.docx

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fe3o4sio2纳米粒子担载漆酶后的cu2修饰及酶学性质研究word格式论文.docx

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fe3o4sio2纳米粒子担载漆酶后的cu2修饰及酶学性质研究word格式论文

2.4 固定化漆酶修饰 ········································································· 132.5 修饰后固定化漆酶相对活性的测定 ················································· 132.6 铜离子修饰固定化漆酶的酶学性质研究 ··········································· 142.6.1 最适 pH 值考察142.6.2 最适温度考察 ········································································ 142.6.3 固定化漆酶降解性能评价 ························································· 142.7 样品的表征方法 ········································································· 142.7.1 扫描电镜分析（SEM）142.7.2 比表面积—孔径分析（BET）14第 3 章低固载量固定化漆酶的铜离子修饰 ··············································· 153.1 前言························································································· 153.2 结果与讨论················································································ 153.2.1 漆酶在 Fe3O4/SiO2 载体上固载量的考察153.2.2 铜离子浓度对固定化漆酶(低固载量)活性的影响···························· 163.2.3 修饰后固定化漆酶的 SEM 分析183.2.4 修饰后固定化漆酶的 BET 分析213.3 本章小结··················································································· 22第 4 章修饰后固定化漆酶的酶学性质研究 ··············································· 234.1 前言························································································· 234.2 结果与讨论················································································ 234.2.1 最适 pH 考察234.2.2 最适温度考察 ········································································ 244.2.3 铜离子修饰后的固定化漆酶的储存稳定性···································· 254.2.4 普施安脱色操作稳定性的研究··················································· 254.3 本章小结··················································································· 27 结论 ······························································································ 28 参考文献 ··························································································· 29攻读硕士学位期间发表的学术论文·························································· 36哈尔滨师范大学学位论文原创性声明······················································· 37哈尔滨师范大学