fas介导sle患者foxp3cd4cd25treg细胞凋亡的研究word格式论文.docx

To explore the role of fas apoptosis signal transduction pathway in the abnormal apoptosis of Foxp3+CD4+CD25+ Treg in SLE patients.MethodsTwenty-five patients with active SLE and twenty patients with remission of SLE were included in this study. Twenty normal volunteers were also enrolled as control group. Expression of fas on peripheral blood Foxp3+CD4+CD25+Treg cells were detected by flow cytometric method. The expression rate of Foxp3 on CD4+CD25+T cells was also analyzed.ResultsThe expression rate of fas on Foxp3+CD4+CD25+Treg cells in active SLE group was higher than that in the remission group [(23.72±2.35)% VS (14.0±2.07)%. P=0.003], and healthy controls [(23.72±2.35)% VS (10.13±1.21)%,P 0.001]. While there were no statistical differences between the remission patients and healthy controls. The expression of fas on the Foxp3+CD4+ CD25+Treg was positively correlated with the SLEDAI score (r=0.336, P 0.05).The expression rate of Foxp3 on CD4+CD25+T cells in active SLE group was significantly lower than that in remission SLE group [(2.83±0.30)% VS (5.38±0.63)%, P=0.008], and healthy controls [(2.83±0.30)% VS (8.12±0.70)%,P 0.001]. Meanwhile the Foxp3 expression on CD4+CD25+T cells of remission group was also lower than that of the control group (P=0.04). The expression of Foxp3 on CD4+CD25+T cells was negatively correlated with the SLEDAI score (r=-0.581, P 0.001).ConclusionThere is high expression of fas on peripheral blood Foxp3+ CD4+ CD25+ Treg cells in active SLE patients. Abnormal apoptosis of Foxp3+ CD4+ CD25+ Treg mediated by the fas apoptosis signal transduction pathway may be one of the pathogenic mechanisms in SLE.Keywords: lupus erythematosus, systemic; foxp3 + CD4 + CD25 + Treg; apoptosis; fas.中英文对照索引缩写词英文全称中文全称SLEsystemic lupus erythematosus系统性红斑狼疮Tregregulatory T cell调节性 T 细胞Foxp3forkhead /winged helix transcription fatorP3叉状头/翅膀状螺旋转录因子 3FCMflow cytometry流式细胞术PBMCperipheral blood mononuclear cells外周血单个核细胞SLEDAIsystemic lupus erythemato