11.基因分析的基本策略-新-英.pptVIP

Chapter 11 基因分析的基本策略 Basic Methods of Gene analysis 第一节 DNA的定性定量分析 (analysis of DNA) DNA定性定量分析方法： DNA序列测定(DNA sequencing) Southern杂交(southern blot) PCR技术(PCR technique) 一、DNA序列测定(DNA sequencing) Application of DNA sequencing : Gene separation、construction of recombinant vector、synthesis of gene fragment、etc. Human genome project (HGP) and Progress of Model Organism Genome Gene structure and function and the relation of gene and diseases The results of DNA sequencing：primary structure of DNA Basic methods dideoxy chain termination (the Sanger method) Chemical cleavage sequencing (Ma