杨荣武分子生物学课件Week4modification.pptVIP

Enzymes for manipulating DNA *** Buffers and solution conditions*** I. DNA polymerases III. Kinase and alkaline phosphatase IV. Nucleases V. Topoisomerase DNA polymerases--making copies, adding labels, or fixing DNA E. coli DNA polymerase I --the classic DNA polymerase Moderately processive polymerase 3-5 proof-reading exonuclease 5-3 strand-displacing (nick-translating) exonuclease Used mostly for labelling DNA molecules by nick translation. For other purposes, the Klenow fragment is usually preferred DNA polymerases Klenow fragment --the C-terminal 70% of E. coli DNA polymerase I; origi