铁皮石斛多糖.docVIP

西南林业大学 本科毕业（设计）论文 （2013 届） 题 目 组培铁皮石斛多糖的提取及其抗氧化性研究(1) 教学院系 林学院 专 业 食品科学与工程 学生姓名 董 燕 平 指导教师 刘 云（实验师） 唐军荣（实验师） 评 阅 人 二0一三年一月十四日 组培铁皮石斛多糖的提取及其抗氧化性研究(1) 董燕平 (西南林业大学林学院，云南 昆明，650224) 摘 要：本试验通过对从组培铁皮石斛中提取出的多糖及维生素c进行了总抗氧化能力的测定和对不同自由基清除率的测定，结果表明，不同样品均具有一定的抗氧化能力，组培铁皮石斛多糖对羟基自由基、超氧阴离子自由基和1,1-二苯基-2-苦基苯肼自由基(DPPH)均具有良好的清除作用。其中，加入组培铁皮石斛多糖60μg时，其对羟基自由基清除率能达到91.4%，对超氧阴离子自由基的抑制率最高达47.08%；当加入组培铁皮石斛多糖700μg时，对DPPH的清除率达到51.91%。 关键词：组培铁皮石斛；多糖；提取；抗氧化 Extraction of Polysaccharides from Tissue Cultivation of Dendrobium Candidum and Research of Its Antioxidation(1) DONG Yan-ping (Southwest forestry University Forest Institute,Kunming,Yunnan 650224) Abstract:In this experiment,the polysaccharides was extracted from Tissue cultivation of Dendrobium Candidum,then the total antioxidation ability and the scavenging rate for different free radical of polysaccharides of Tissue cultivation of Dendrobium Candidum and Vitamin C were determined.The result showed that different samples had must of antioxidation abilities,and the the polysaccharides of Tissue cultivation of Dendrobium Candidum had favourable scavenging action for hydroxyl free radical, superoxide anion free radical and DPPH.When the adding quantity of Polysaccharides of Tissue cultivation of Dendrobium Candidum was 60μg,its scavenging rate to hydroxyl free radical reached 91.4% and its scavenging rate to superoxide anion free radical reached 47.08%;when the adding quantity of Polysaccharides of Tissue cultivation of Dendrobium Candidum was 700μg,,its scavenging rate to DPPH reached 51.91%. Keywords : Tissue cultivation of Dendrobium Candidum; Polysaccharides; Extraction; Antioxidation 目 录 1 前言 1 1.1 铁皮石斛简介 1 1.2 铁皮石斛多糖简介 1 1.3 铁皮石斛国内外研究现状 2 1.4 试验目的和意义 3 1.4.1 试验目的 3 1.