PTEN基因在小鼠胚胎着床过程中作用的实验研究.docVIP

PTEN基因在小鼠胚胎着床过程中作用的实验研究 作者：陈晓玲,杨戎,贾咏存,刘孝云,魏莎莉 单位：重庆医科大学公共卫生学院生殖生物学研究室,重庆, 400016 提要:目的　探讨PTEN基因小鼠胚胎着床过程中的作用。方法　实时荧光定量聚合酶链反应( real-time fluorescence quantitative PCR,FQ-PCR)分别检测未孕及孕1、3、4、5、7 d(分别记为d0、d1、d3、d4、d5、d7)小鼠子宫内膜PTEN基因mRNA的表达和子宫角注射反义寡核苷酸观察胚泡着床数。结果　FQ-PCR测得妊娠的子宫内膜组织PTEN的表达高于未妊娠的子宫内膜组织,且随着妊娠天数的增加呈现逐渐增加的趋势,到妊娠第5天达到最高。子宫角注射PTEN反义寡核苷酸后胚泡着床数明显减少。结论　PTEN在妊娠早期子宫内膜持续表达,可能参与了胚泡着床。 　　关键词: PTEN;子宫内膜;植入;FQ-PCR;子宫角注射 　　中图法分类号:R-33;R321. 3;R394. 2　　　文献标识码:A PTEN gene expression in mouse endometria increases during embryo implantationCHEN Xiao-ling,YANG Rong,JIA Yong-cun,LIU Xiao-yun,WEI Sha-li(Laboratory ofReproductive Biology,ChongqingMedicalUniversity,Chongqing 400016,China) 　　Abstract:Objective　To investigate the effectofPTEN gene in themouse uterusduring embryo implanta-tion.Methods　Real-time fluorescence quantitative PCR(FQ-PCR)was used to detect PTEN mRNA ex-pressed in the endometria of the nonpregnantmice and the late pregnantmice(day1,day3,day4,day5 andday 7),with 20 mice sacrificed ateach fixed day. Outofanother20 3-day regnantmice,ten received PTENantisense oligonucleotide atthe horn ofuterus and ten received normalsaline to countthe blastocysts atpregnantday 8.Results　ThePTENmRNA/beta;-actinmRNA in pregnantmicewashigher than thatofnonpregnantmice, gradually hoisted as days passed by,and reached the highest atpregnantday 5. The number ofblastocysts inthemice that received PTEN antisense oligonucleotidewas fewer than that received normal saline.Conclusion　PTEN persistently expresses in mouse endometria during the early pregnancy and maybe participate in theregulation process ofmouse blastodyst implantation. 　　Key words:PTEN;endometrium;implantation;real-time fluorescence quantitative PCR;intrauterine injection 　　PTEN是位于人类染色体的10q23的肿瘤抑制基因,编码一种双特异性的蛋白磷酸化酶,能够脱去丝氨酸/苏氨酸和酪氨酸残基的磷酸[1]。序列分析表明,该蛋白质与张力蛋白和辅助蛋白有很大的同源性[2],促进细胞凋亡的发生。它在体外同时具有脂质磷酸酶和蛋白水解酶的活性。近年的研究表明,它在胚胎发育、着床,细胞迁移,和细胞凋亡等中发挥重要的作用[1, 3]。胚胎着床和胎盘形成过程会发生一系列的细胞间相互作用,细胞增值,侵入以及细胞凋亡等,导致着床点处组织重建。细胞凋亡可能在胚胎发育和多余细胞清除方面起关键性的作用。本