sHLAG检测及胚胎植前筛选.docVIP

sHLAG检测及胚胎植前筛选 作者：孙新六1,刘秀兰2 单位：1. 泰山医学院附属泰安医院中心实验室，山东 泰安 271000;2.泰山医学院附属泰安医院妇产科，山东 泰安 271000 【摘要】目的 探讨胚胎植前筛选以提高胚胎移植成功率。方法 单胚培养体外受精胚胎，ELISA方法检测移植成功组与失败组胚胎培养上清液sHLAG水平，并比较培养上清中sHLAG与胚胎HLAG mRNA表达的相关性。结果 胚胎移植成功组sHLAG水平显著高于未成功受孕组;sHLAGgt;10 ng/ml移植组受孕成功率60.9%,sHLAGlt;5 ng/ml成功率11.5%。结论 sHLAG是植前筛选良好指标，能提高胚胎移植成功率。 【关键词】 可溶性人白细胞抗原G;胚胎移植;植前筛选 Detecting of sHLAG and selection for preimplantation embryosSUN Xinliu1, LIU Xiulan2(1.the Central Laboratory of Taian Central Hospital, Taian 271000, China; 　　2.the Dept. of Obstetrics and Gynecology of Taian Central Hospital,Taian 271000, China) 　　Abstract:Objective:Investigating the selection for preimpantation embryos to emhance the rate of successful embryo implantation.Methods：The embryos were cultured by one embryos every hole,we detected the concentration of sHLAG in supernatant cultures of embryos in successful pregnant group and unsuccessful pregnant group respectively,compared the correlation between sHLAG in supernatant cultures of embryos and expression of HLAG mRNA in embryos.Results： the sHLAG was significantly higher in pregnant group than in nonpregnant group,the rate of successful pregnant was 60.9% in group of sHLAG up 10ng/ml and 11.5% in group of sHLAG under 5ng/ml respectively. Conclusion：sHLAG was an ideal indictor of selection for preimpantation embryos and can echance the rate of successful embryo implantation. 　　Key words:sHLAG;embryo implantation;selection of preimpantation embryos 　　人白细胞非经典抗原G (human leukecyte antigenG,HLAG)是HLAI类抗原，主要分布于绒毛外滋养层细胞[1]，sHLAG是可溶性HLAG,存在于孕妇外周血清中，sHLAG亦可由受精胚胎表达，与临床密切相关[2]。本实验旨在研究以sHLAG为指标植前筛选移植胚胎以期提高体外受精胚胎移值(IVFET)成功率。 　　1 材料与方法 　　1.1 试剂及实验对象 　　受精胚胎由本院生殖中心提供;RTPCR试剂盒购自TAKARa公司;胚胎培养基购自丹麦Medicult公司;sHLAG试剂盒购自Biovendor公司。 　　1.2 胚胎制备及培养 　　促超排卵、取卵、卵母细胞收集及体外受精均由生殖中心按常规操作完成。受精胚胎置于8孔培养板中，每孔放置一个胚胎，并用1 ml Denmark培养基培养48 h,镜下挑选形态完好的胚胎，转入新鲜培养基中继续培养72 h,筛选合格胚胎，并收集培养上清。合格胚胎筛选标准：分裂球数目 ge; 6个;分裂球形态规则;碎片lt;5%;卵裂球大小均匀，透亮。 　　1.3 胚胎移植 　　由生殖中心按常规程序完成，胚胎移植对象均为30~35周岁女性，