本科毕业论文：一株产红色素真菌的初步鉴定及色素提取与稳定与本科毕业论文：苏轼人物传论的思想内涵合集.docVIP

本科毕业论文：一株产红色素真菌的初步鉴定及色素提取与稳定与本科毕业论文：苏轼人物传论的思想内涵合集 本科毕业论文：一株产红色素真菌的初步鉴定及色素提取与稳定 本科毕业论文：一株产红色素真菌的初步鉴定及色素提取与稳定目录 摘要1Abstract2引言31 菌株的鉴定51.1 材料、试剂与仪器51.2 方法61.3 结果与分析61.4 小结与讨论７2 色素提取工艺的研究７2.1 材料、试剂与仪器７2.2 方法８2.3 结果与分析102.4 小结与讨论133 色素稳定性的研究133.1 材料、试剂与仪器133.2 方法143.3 结果与分析153.4 小结与讨论20结语20参考文献21致谢24附录25 摘要 真菌色素是一种极具应用潜力的食用色素。本文针对一株产红色素的真菌A11625M，对菌株进行了鉴定、色素提取工艺和色素稳定性方面的研究。实验结果表明：该菌株通过形态学鉴定为酒红拟青霉(Paecilomyces vinaceus)。菌株所产红色素，其最大吸收波长为510 nm；色素提取时，以鲜菌丝直接浸提，最佳提取剂为丙酮，提取时间为240 min，提取温度为30 ℃；提取时对菌丝进行超声波破碎预处理有助于色素的提取。该色素在中性和弱酸弱碱 条件下的稳定性较好；温度对色素稳定性的影响较小；糖类对色素稳定性的影响不明显；金属离子Mg2+、Cu2+、Na+、K+、Fe3+、Zn2+、Fe2+、Ca2+对色素稳定性的影响较小；过氧化氢对色素稳定性的影响较大，亚硫酸钠对色素稳定性的影响较小，抗坏血酸能提高色素的稳定性。 关键词：真菌；红色素；鉴定；提取；稳定性 Abstract Fungal pigments were potential resource for natural food colorant. In this article, an unknown fungus strain A11625M, which can produce red pigment,was studied on its identification, pigment extraction and the pigment stability.This fungal strain was identified as Paecilomyces vinaceus by morphological characters. The maximum absorption light spectrum lengh of the pigment, which produced by the strain, was 510 nm. Fresh mycelium was more suitable for pigment extraction than dry mycelium, the best extraction solvent was acetone, and the optimal extraction time was 240 minutes, the optimal extraction temperature was 30 ℃. In addition, crushing the mycelium could be helpful for pigment extraction by ultrasonic. The pigment was stable in neutral, weak acidic and alkalescent condition. Temperature had the little effect on the stability of the pigment. Carbohydrate had no influence on the pigment. Metallic ions, such as Mg2+, Cu2+,Na+, K+, Fe3+, Zn2+, Fe2+, Ca2+ had the little influence on the pigment; H2O2 influenced the pigment significantly; The Na2SO3 had little influence on the pigment; The ascorbic acid could improve the stability of the pigment. Keywords: Red pigment; fungus; identification; extraction; stability 引言（1）天然色素概况 色素也叫着色剂，是改变物品反射或者透射光颜色的物质。根据不同的用途可分为染料、颜料、食用色素[1]。食用色素是一类用于