基于cds-pamamgnps膜电化学发光免疫传感器构建及其对毒品超灵敏检测-construction of electrochemiluminescence immunosensor based on cds - pamgnps film and its ultra-sensitive detection of drugs.docx

基于 CdS-PAMAM/GNPs 膜的电化学发光免疫传感器的构建及其对毒品的超灵敏检测英文摘要Construction of electrochemiluminescence immunosensor based on CdS-polyamidoamine/gold nanoparticles film and its ultrasensitive detection of drugsAbstrastDrugs are substances which can make people addict to them. Usually, they are divided into two categories of narcotic drugs and psychotropic drugs. Morphine (MOP) is the main alkaloid of opium and ketamine (KET) belongs to the non-opium of anesthesiology drugs. Both of them have strong anesthetic and analgesic effects on the central nervous system. On the one hand they bring serious damages to social and drug addicts; on the other hand they relieve pain in surgical procedures. So it has very important realistic significance to detect the morphine and ketamine contents in drug addicts and patients who were injected morphine and ketamine. In this paper, well-distributed CdS/PAMAM QDs was prepared with PAMAM as a template and based on CdS-polyamidoamine/gold nanoparticles film we have developed a novel ECL immunosensor. Combining high sensitivity of electrochemiluminescence (ECL) and selectivity of immunoreaction, a further application of the ECL immunosensor was investigated. It has the remarkable features such as high sensitivity, good selectivity and high stability. This ECL immunosensor was used to detect morphine and ketamine and the satisfied result was obtained. The major work and results are as followed:In the first part of this paper, the CdS-PAMAM/GNPs ECL sensor was configurated and its performance was studied. We had successfully prepared well-distributed CdS/PAMAM QDs with PAMAM as a template. Transmission electron microscopy (TEM) figure showed that CdS-PAMAM QDs was about 4 nm in size and had a good dispersibility and stability. The numerous reactive groups on the surface of dendrimers can render the CdS/PAMAM QDs to assemble firmly onto the electrodeposited gold nanoparticles film to configurate CdS-PAMAM/GNPs/Au electrode. The film electrode was characte