基底拉伸和压缩加载对肝癌细胞接触抑制丧失生长行为影响-effect of basal stretch and compressive loading on contact inhibition and los of growth behavior of hepatocellular carcinoma cells.docx

ABSTRACTLiver cancer cells in vitro cultivation of two-dimensional there is an obvious phenomenon of loss of contact inhibition, which is the outstanding performance for the proliferation of malignant cells. Contact inhibition of cell growth is a result of interaction between social behavior, and goes beyond a single cell line and to groups linked to the cell skeleton network system, and cell social behaviour and its regulation closely related. Among them, to a skeleton structure as the carrier of the pre-tension on cell growth and proliferation activities, has a direct role in the regulation and control. Cytoskeleton structure of tumor cells with shortcomings, the resulting tension pre-abnormal regulation of tumor cells and the loss of contact inhibit is otherwise related. This paper through mechanical means loading the growth substrate of liver cancer cell for contraction to stimulate the stretch, observing pre-tension in the cytoskeleton. To link cells and cell proliferation of research findings revealed the tumor cells causes loss of a inhibition contact with a certain sense.Object Through researching relationship between distribution of E-cadherin, expression ,cell movement and skeleton pre-tension among the liver cells and liver cancer cells, we explore the loss of contact inhibition mechanism in liver cancer cells.Methods With microscopic morphology, image analysis software, immune staining, confocal laser microscopy techniques, such as research tools, respectively, we dectect different growth density of liver cancer cells and liver cells morphological characteristics, cytoskeleton deformation ability to change and movement, and E-cadherin expression and changes in distribution and quantitative analysis. The cell synchronization, MTT and flow cytometry, and other experimental technology were used in mechanical drawing and contraction after the liver cancer cells to stimulate change and the proliferation framework for testing and quantitative analysis.Result