家蚕细胞系bmn凋亡过程中相关基因的转录特征分析-analysis of transcription characteristics of related genes during apoptosis of silkworm cell line bmn.docx

家蚕细胞系（BmN）凋亡过程中相关基因的转录特征研究英文摘要The study on apoptosis characteristics of related genes transcription of silkworms (Bombyx mori) cell lines (BmN)AbstractThe silkworm, Bombyx mori, is an important economic insect, is a typical pattern of insects. Targeted research on apoptosis of Bombyx mori has been reported, for example, the blood of B. mori can play the role of anti-apoptotic effects, the molting hormone can induce cell apoptosis. But compared with the Nematode, Drosophila, human and other model organisms, the study on the apoptosis pathway in B. mori is still at the starting stage. The study on apoptosis in silkworm ovarian cell lines (BmN) is rarely reported. In this study, at the background of induction factors (H2O2, virus) in vitro, we do the research on the cell morphology of apoptosis and gene expression level differences by technologies like morphological observation, agarose gel electrophoresis and quantitative real-time PCR. We also explore the role of two kinds of ace of B. mori in the process of apoptosis, the results are as follows.Apoptosis was induced by H2O2. BmN cells were induced by H2O2 at 0.100 mol/L for 3 h, the features of apoptosis can be observed in the optical and fluorescence microscopy, ladder shape strap can be obviously observed by agarose gel electrophoresis after 24 h induction. The QRT-PCR results showed that, the expression of Bombyx mori, telomerase gene, Bmtrt decreased; the expression of apoptosis-related genes of B. mori Bmcaspase-5 gradually increased. Compared with the control group, 41.942 times increased at 24 h; the expression of Bmp53 gene decreased at first and then gradually increased and 1.592 times increased at 24 h. The results showed that after H2O2 induction, the B. mori apoptosis-related genes have emerged characteristic expression, the relationship between genes in the process of apoptosis will be studied in the future.Apoptosis was induced by Bombyx mori nuclear polyhedrosis virus, BmNPV.After the infection by BmN