pBK-CMV哺乳动物表达载体说明.pdfVIP

pBK-CMV 编号 载体名称 北京华越洋生物VECT6037 pBK-CMV pBK-CMV载体基本信息 载体名称: pBK-CMV Phagemid vector ；pBK-CMV 质粒类型: 真核原核双系统表达载体；克隆载体；噬粒载体 高拷贝/低拷贝: 高拷贝 克隆方法: 多克隆位点，限制性内切酶 启动子: 原核启动子lac ；真核启动子CMV 载体大小: 4518bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: -- 载体抗性: 卡那霉素 筛选标记: 新霉素Neomycin 和卡那霉素Kanamycin 备注: 用蓝白斑法筛选原核细胞阳性克隆。 稳定性: -- 组成型/诱导型: 真核组成型表达，原核IPTG诱导型表达 病毒/ 非病毒: 非病毒 pBK-CMV载体质粒图谱和多克隆位点信息 pBK-CMV 载体简介 pBK-CMV Phagemid Vector The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector. In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed. In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of inducer. • Constitutive expression in eukaryotic cells • Inducible protein expression in prokaryotes • Rescued from ZAP Express lambda vector The pBK-CMV phagemid vector is a cloning vector derived from a high-copy-number pUC-based plasmid. This vector allows expression in both eukaryotic and prokaryotic systems. Eukaryotic expression is driven by the cytomegalovirus (CMV) immediate early promoter in the pBK-CMV phagemid vector. Stable clone selection in eukaryotic cells is made possible with G418 by the presence of the neomycin- and kanamycin-resistance gene,