GADD45G在雄性性别决定中的功能课件.pptVIP

GADD45G Functions in Male Sex Determination by Promoting p38 Signaling and Sry Expression GADD45G通过促进p38信号和Sry表达在雄性性别决定中起作用 一、摘要 Gadd45g display complete male-to-female sex reversal. 二、材料和方法 1.Animals, Staging, and Dissection 三、结果 1. Gadd45g突变体鼠显示了雄性到雌性的性逆转 1. Gadd45g突变体鼠显示了雄性到雌性的性逆转 The early male gonad speci?cation is permanently interrupted in Gadd45g-/-. 2. Gadd45g对于正常Sry表达是必不可少的 2. Gadd45g对于正常Sry表达是必不可少的 2. Gadd45g对于正常Sry表达是必不可少的 2. Gadd45g对于正常Sry表达是必不可少的 2. Gadd45g对于正常Sry表达是必不可少的 2. Gadd45g对于正常Sry表达是必不可少的 2. Gadd45g对于正常Sry表达是必不可少的 2. Gadd45g对于正常Sry表达是必不可少的 3. Gadd45g与Sry和Sox9共表达 3. Gadd45g与Sry和Sox9共表达 3. Gadd45g与Sry和Sox9共表达 3. Gadd45g与Sry和Sox9共表达 3. Gadd45g与Sry和Sox9共表达 4.Sry基因脱甲基化在Gadd45g突变体不被影响 4.Sry基因脱甲基化在Gadd45g突变体不被影响 4.Sry基因脱甲基化在Gadd45g突变体不被影响 4.Sry基因脱甲基化在Gadd45g突变体不被影响 4.Sry基因脱甲基化在Gadd45g突变体不被影响 5. Gadd45g对MAP激酶信号是必需的 5. Gadd45g对MAP激酶信号是必需的 5. Gadd45g对MAP激酶信号是必需的 5. Gadd45g对MAP激酶信号是必需的 5. Gadd45g对MAP激酶信号是必需的 5. Gadd45g对MAP激酶信号是必需的 四、讨论 四、讨论 四、讨论 四、讨论 四、讨论 Map3k4 and Map3k1 cause male-to-female sex reversal and testis abnormalities, respectively Activation of MAPK signaling is one of the best-documented cellular functions of GADD45 family members GADD45G speci?cally activates MAP3K4； p38 signaling in various cell types； GADD45G may also regulate MAPK signaling during male gonad development. (A) Western blot analysis of gonads and mesonephroi of male wild-type embryos at 18 ts， p38 is robustly phosphorylated MAPK cascade is functionally active during early gonadal development (B) Electroporation of Gadd45g increased phospho-p38； GADD45G ； drive MAPK signaling in gonadal cells p38 signaling in Sry regulation dissected at 7/8 ts；SB202190 inhibition of Sry expression； SF1， slightly induced culture of genital ridge explants Anisomycin activate p38 upregulated Sry expression GADD45G regulates Sry via p38 MAPK signaling. downstream of p38 MAPK signaling ，regulate Sry disrupted GATA4 and FOG2 interaction The transcriptional activity of GATA4 ， regu