配伍对冠心丹参方抗急性心肌缺血作用差异的研究.pdfVIP

配伍对冠心丹参方的抗急性心肌缺血作用的差异研究 1,2 1 冯群 ，孙蓉 （1. 山东省中医药研究院，山东济南250014 ；2.天津中医药大学，天津 300193 ） 摘要：目的 比较冠心丹参方中的药物有效组分不同配伍对垂体后叶素致大鼠急性心肌缺血模型的保护作 用。方法 采用垂体后叶素造成急性心肌缺血模型，观察药物对大鼠心电图ST 段、T 波的位移值，急性心 肌缺血梗死范围及心肌组织的病理学检查，比较组分配伍对抗心肌缺血类中药发挥功效的差异。结果 药 物不同组分配伍均能明显对抗垂体后叶素引起的急性心肌缺血的心电图 ST 段、T 波的变化，能降低急性 心肌缺血梗死范围、保护心肌细胞。其中冠心丹参方组对心肌缺血的保护作用最佳。结论 冠心丹参方中 药物不同组分配伍具有显著对抗垂体后叶素致大鼠急性心肌缺血的作用，效应强度为：冠心丹参方丹参+ 三七丹参+ 降香丹参三七+ 降香。有效组分配伍的药效结果符合中药方剂配伍原则。 关键词：冠心丹参方；组分配伍；垂体后叶素；急性心肌缺血 Differential Study of protection of Guanxin Danshen Formula by Combinations on Acute Myocardial Ischemia in Rats FENG Qun1,2, SUN Rong1 （1. Shandong academy of Chinese medicine, Ji’nan 250014, China; 2. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China） ABSTRACT: OBJECTIVE To compare the protective effects of Guanxin danshen formula (GDF) by combinations with effective components on pituitrin-induced acute myocardial ischemia in rats. METHODS The rat model of acute myocardial ischemia was established by injecting pituitrin into sublingual vein. The effects of medicine on electrocardiogram (ECG) ST segment and T wave in rats in different time after pituitrin injection were observed. Myocardial infarction size (MIS) of model rats was calculated and pathological examination of myocardial tissue was performed. These indexes were used to compare the difference of protective effects of GDF by combinations with effective components. RESULTS Changes of ECG ST segment and T wave of pituitrin-induced acute myocardial ischemia rats were obviously inhibited by combinations with effective components of GDF. MIS was reduced and myocardial cells were protected. The GDF group was the best to protect myocardial ischemia. CONCLUSION GDF by combinations with effective components can significantly inhibit acute myocardial ischemia in rats caused by pituitrin. The sequence of potency is as f