hnf4a靶向激活mmp14促进神经母细胞瘤侵袭 转移及血管形成-hnf4a targeted activation of mmp 14 promotes the invasion and metastasis of neuroblastoma and angiogenesis..docx

独创性声明 本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。 尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过 的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识 到本人将承担本声明引起的一切法律后果。 学位论文作者签名： 日期： 年 月 日 学位论文版权使用授权书 本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关 部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将 本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段 保存和汇编本学位论文。 保密□，在 年解密后适用本授权书。 本论文属于 不保密□。 （请在以上方框内打“√” ） 学位论文作者签名： 指导教师签名： 日期： 年 月 日 日期： 年 月 目 录 缩略词表 ·························································································································· 1 研究背景 ·························································································································· 3 中文摘要 ·························································································································· 7 ABSTRACT 10 第一部分 神经母细胞瘤组织和细胞株中 HNF4 a 和 MMP-14 表达相关性研究 14 实验材料 ······················································································································14 实验方法 ······················································································································18 结 果 ·························································································································26 讨 论 ·························································································································37 参考文献 ······················································································································38 第二部分 HNF4a 靶向激活 MMP-14 调控神经母细胞瘤侵袭、转移和血管形成活性 41 实验材料 ······················································································································41 实验方法 ······················································································································46 结 果 ·························································································································59 讨 论 ············