il6jakstat3与tgfβsmad通路介导肺癌细胞上皮间充质转化过程的交互作用机制研究-study on interaction mechanism of il 6 jak stat 3 and tgf β smad pathway mediated epithelial mesenchymal transition in lung cancer cells.docx

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il6jakstat3与tgfβsmad通路介导肺癌细胞上皮间充质转化过程的交互作用机制研究-study on interaction mechanism of il 6 jak stat 3 and tgf β smad pathway mediated epithelial mesenchymal transition in lung cancer cells.docx

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il6jakstat3与tgfβsmad通路介导肺癌细胞上皮间充质转化过程的交互作用机制研究-study on interaction mechanism of il 6 jak stat 3 and tgf β smad pathway mediated epithelial mesenchymal transition in lung cancer cells

IL-6/JAK/STAT3与TGF-β/Smad通路介导肺癌细胞上皮-间充质转化过程的交互作用机制研究英文摘要Cross-talkbetweenIL-6/JAK/STAT3andTGF-β/Smadsignalingduringepithelial–mesenchymaltransitioninlungcancercellsAbstractBackgroundandObjective:Lungcanceristheleadingcauseofcancerdeathworldwide.Understandingthemechanismoflungcancermetastasisisverycrucial,sinceover90%ofmortalityfromsolidtumorsisattributabletometastases.Asakeystepintheearlystageofcancermetastasis,epithelial-mesenchymaltransition(EMT)isorchestratedbyseveralsignalingpathways,includingTGF-β/SmadandJAK/STAT3signaling.TheaimofthisstudyistodetectthemechanismsofEMTinducedbythecross-talkbetweenTGF-β/Smad3andJAK/STAT3signalingpathwaysinnon-smallcelllungcancer(NSCLC)cells.Methods:(1)NSCLCcellsweretreatedwithTGF-β1,andthecellmorphologywasexaminedandphotographedusingaphase-contrastmicroscope.LevelsofSnailindifferenttimeperiodafterTGF-β1treatmentweremonitoredbyquantitativereal-timePCR(qRT-PCR)andwesternblot.(2)TheexpressionofSmad3andthephosphorylationofSmad3wereassessedbywesternblot.AftertreatedwiththespecificinhibitorofSmad3phosphorylationorTGF-β1,themorphologyofcellswasexaminedandphotographed,thelevelsofmolecularmarkersofEMT,Snail,Smad3andp-Smad3wereexaminedbywesternblot.(3)CellswereincubatedwiththespecificinhibitorofJAK/STAT3signalingAG490,andtheexpressionofStat3andp-Stat3weredetectedbywesternblot.AftertreatmentwithAG490and/orTGF-β1,wedeterminedtheexpressionofSmad3,p-Smad3andSnailbywesternblotandmeasuredtheexpressionofMMP-2usingqRT-PCR.Furthermore,weconstructedaluciferasereportergenethatharboringthepromoterregionofPAI-1anddetectedtheactivityofthereportergene.AndthemigrationandinvasionoflungcancercellsweredeterminedbywoundhealingandTranwellassays.(4)ExogenousadditionofIL-6tolungcancercells,anddetectedtheexpressionofStat3andp-Stat3bywesternblot.Next,cellswereincubatedwithIL-6英文摘要IL-6/JAK/STAT3与TGF-β/Smad通路介导肺癌细胞上皮-间充质转化过程的交互作用机制研究and/orTGF-β1,thelevelsofEMTmolecularmarkers,Smad3,p-Smad3,Smad4andSnailwereassessedbywesternblot.Thelungcancercellmigrationandinvasionw