il13ra2抗原肽致敏树突状细胞对人脑胶质瘤及其肿瘤干细胞的杀伤效应研究-study on killing effect of dendritic cells sensitized with il 13 ra 2 antigen peptide on human glioma and its tumor stem cells.docx

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il13ra2抗原肽致敏树突状细胞对人脑胶质瘤及其肿瘤干细胞的杀伤效应研究-study on killing effect of dendritic cells sensitized with il 13 ra 2 antigen peptide on human glioma and its tumor stem cells.docx

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il13ra2抗原肽致敏树突状细胞对人脑胶质瘤及其肿瘤干细胞的杀伤效应研究-study on killing effect of dendritic cells sensitized with il 13 ra 2 antigen peptide on human glioma and its tumor stem cells

结果 ·············································································· 64讨论 ·············································································· 74参考文献 ········································································ 79全文小结 ·············································································· 82综述··················································································· 83 附录: 攻读博士期间发表文章 ··················································101 致谢···············································································102英文缩略词表英文缩写英文全称中文名称CNSCentral nervous system中枢神经系统DCdendritic cell树突状细胞NKnatural killing cell自然杀伤细胞APCantigen presenting cell抗原呈递细胞CTLcytotoxic T lymphaocyte细胞毒性 T 淋巴细胞BTSCBrain tumor stem cell脑肿瘤干细胞ELISAEnzyme-linked immunpsorbent assay酶联免疫吸附测定FCMflow cytometry流式细胞术CCK-8cell counting kit-8CCK-8 试剂盒FITCfluorescein isothiocyanate异硫氰酸荧光素Bfgfbasic fibroblast growth factor碱性成纤维生长因子IL-4Interleukin-4白细胞介素 4ECFEpidermal growth factor表皮生长因子GM-CSFgranulocyte-macrophage colony - stimuating factor粒-巨噬细胞集落刺激因子IFN-γinterferon-gamma干扰素-γAAbsorbance吸光度ODOptical density光密度MHCmajor histocompatibility complex主要组织相容性复合物HLAhuman leukocyte antigen人类白细胞抗原FCSfetal calf serum胎牛血清PBSPhosphate-buffered saline磷酸盐缓冲溶液DMEMDulbecco’s modified Eagle medium培养基BSABovine serum AGPumin牛血清白蛋白DMSODimethyl sulfoxide二甲基亚砜Gram克mgMilligram毫克μgMicrogram微克ngNanogram纳克LLitre升mlMillilitre毫升μlMicrolitre微升Hour小时minMinute分钟UUnit单位rpmRevolutions per minute每分钟转速SD 95% CIstandard deviation95% confidence interval标准差95%置信区间IL-13Ra2 抗原肽致敏树突状细胞对人脑胶质瘤及其肿瘤干细胞的杀伤效应研究博士研究生：王莹博士生导师：雷霆教授中文摘要目的胶质瘤是中枢神经系统(central nervous system, CNS)最常见的恶性肿瘤，起源于神经胶质细胞，包括星形细胞系瘤，少突胶质细胞系肿瘤，混合性胶质细胞瘤，室管膜瘤等。据统计，胶质瘤约占颅内原发性肿瘤的 35.26-60.96%(平均 44.69%)。由于肿瘤分化不全呈侵袭性生长，手术切除不能够彻底，预后较差。即使进行放化疗也都很难有效根除，病人平均生存期约 14.6 个月，一般不到两年，极大威胁了人们的生命健康。因此，寻找新的胶质瘤治疗方式成为近 20 年来研究的热点。随着对中枢神经系统及胶质瘤免疫特性认识的逐渐深入，生物免疫治疗已成为第四种治疗模式，是治疗颅内肿瘤最有前景的新疗法。DC 疫苗由于安全，有效及低毒，是胶质瘤免疫治疗的重点。树突状细胞(dendritic cells,DCs)是