il17a对肺纤维化大鼠肺组织炎症的调控分析-regulation and analysis of il17a on pulmonary inflammation in rats with pulmonary fibrosis.docx

IL-17A 含量、肺组织 IL-17A 蛋白的表达分别与 BALF 中性粒细胞数呈正相关；而第 28 天时它们之间的相关性较差。结论：1.本实验通过博莱霉素 诱导成功建立了大鼠肺纤维化模型。2. 肺纤维化的发展中，肺泡巨噬细胞 和肺组织中 IL-17A 表达及分泌均升高，在肺泡炎阶段尤为明显。3. IL-17A 可能促进了肺泡炎的发展，并且在肺纤维化的发生、发展病理过程中可能 参与了作用。关键词：肺泡巨噬细胞，IL-17A，博莱霉素，肺纤维化IL-17A’s Regulation on Pulmonary Inflammation of Pulmonary Fibrosis RatsAbstract: Objective: Through observing the expression of IL-17A in rats’ alveolar macrophage whose pulmonary fibrosis was induced by bleomycin, the effect and significance of IL-17A produced in pulmonary fibrosis rats’ alveolar macrophage were explored.Methods: 1. Twenty Wistar female rats (weight 180~200g) were randomly divided into normal saline control group (NS group) and bleomycin-induced pulmonary fibrosis model experimental group (BLM group). And there were ten rats in each group.2. In order to induce replication of pulmonary fibrosis model, the rats of BLM group once received intratracheal injection of 0.2ml bleomycin solution which was diluted with normal saline (5mg/ml).While the rats of NS group were once carried out intratracheal injection with 0.2ml normal saline (1ml/kg).3. Five rats of each group were randomly executed on 7th day and then another five ones were executed on 28th day after the intratracheal injection. Then bronchoaleveolar lavage was perfused, the bronchoalveolar lavage fluids (BALF) were gathered and lung tissues were observed through pathological methods for assessing the degree of pulmonary inflammation and pulmonary fibrosis, the expression of IL-17A protein was detected through the immunohistochemistry. The BALF cells were gathered and then divided into two groups. One group was used to detect the total and differential amount of cells, and also to detect the concentration of TNF-??and IL-17A in BALF through ELISA. Another group was used to separate, purify and cultivate alveolar macrophage(AM). IL-17A was detected through cultivating supernatant in ELISA method. And mRNA expression of IL-17A was detected through RT-PCR by extract