景天属三种植物药的鉴定 总黄酮提取纯化及抗肿瘤活性分析-identification of three plant medicines in sedum extraction, purification and antitumor activity analysis of total flavonoids.docxVIP

density and length/width, were more influenced by habitat, which highly restricted the employment for classification.Through the comparative study of many samples of the 3 plant drugs harvested in different seasons and places, a remarkable relation was found between total flavonoid content and harvest season. The 3 plant medicines all contained the most flavonoids in April (flowering period), and then the flavonoid quantites kept decreasing from April to June or July. However, the content of Sedi Herba and Herba Sedi Linearis began to rise a little after August, while that of Sedi Emarginati Herba continued to drop during autumn. Considerating the flavonoids as the effective components, it seemed that all the 3 plant drugs produced in Hubei Province should have the best quality if reaped in flowering time, and samples collected in the west of Hubei Province usually possessed higher quality than those collected in the east. The method established was simple and fast, having enriched the approache s for the quality evaluation.Study on total flavonoid purification methods of 3 Sedum plant drugs revealed that alkali- solution and acid- isolation method was simple but with a low flavonoid content in the extraction; while the coupling technique of macroporous resin and polyamide absorption column, which could give full play to the advantages of the both, showed a good concentration effect. By this way, the flavonoid content of the extractions of Sedi Herba, Herba Sedi Linearis and Sedi Emarginati Herba respectively reached 68.9%, 54.0% and 51.5%.MTT method was applied to detect the inhibition effects of the extractions ofethyl acetate, n-butyl and total flavonoids of the 3 Sedum plant drugs against HepG2, EC109 and SW480. The results indicated that ethyl acetate and n-but yl fractions possessed certain antitumour effectiveness, while the total flavonoid extractions of each drug had the strongest effectiveness. The sequence of the antineoplastic bioactivity of the 3 extr